Cat assay, Biology

CAT assay is an enzyme assay. CAT stands for the chloramphenicol acetyl transferase, a bacterial enzyme which inactivates the chloramphenicol by acetylating it. CAT assays are many times performed to test the function of a promoter. The gene coding for CAT is linked onto the promoter (transcription control area) from another gene, and the construct is "transfected" into cultured cells. The quantity of CAT enzyme produced is taken to indicate transcriptional activity of the promoter (relative to other promoters which should be tested in parallel). It is simple to perform a CAT assay than it is to do a Northern blot, so CAT assays were common techniques for testing the effects of sequence changes on the promoter function. It is largely supplanted by the reporter gene luciferase.

Posted Date: 8/1/2012 7:15:15 AM | Location : United States







Related Discussions:- Cat assay, Assignment Help, Ask Question on Cat assay, Get Answer, Expert's Help, Cat assay Discussions

Write discussion on Cat assay
Your posts are moderated
Related Questions
How will the urine concentration be affected if the henle''s loop of a person is removed and his PCT is joined directly with DCT?

various changes that occur during concentration of food

Packs, pouches, and cassettes are sometimes wet after completing the sterilization process. This is a problem usually associated with the use of paper or paper/ plastic wraps in t

Typical Form or Reparative Regeneration - Heteromorphosis Sometimes the part which grows back is not the same as that which was lost. The phenomenon is called Heteromorphosis.

When coal is carbonized coke is formed along with coal gas.                             1300 0 C          Coal                                  coke + coal gas

Epididymitis  Acute epididymitis in men less than 35 years old is usually caused by C. trachomatis or, less frequently, N. gonorrhoeae. Older men or those who have had urinary

Question : (a) Briefly describe what are non degradable pollutants? (b) What do you meant by ichthyosarcotoxic fishes and give two examples of ichthyosarcotoxic fish poiso

In the year of 1953, Watson and Crick worked out the 3-D structure of DNA, beginning from X-ray diffraction photographs   taken through Wilkins and Franklin. They decreased which D

Explain the Malthus' Theory about population growth? Darwin's Theory was also based on Thomas Malthus' writings on population growth, in which he explained that populations gro

VITAMIN - A Discovered by Macollum. Also known as Retinol / Antixeropthalmic vitamin / Auxerophthol. It is vitamin for normal vision. It forms retinal pigments rho