Calculate the volumetric substrate utilization rate, Physics

Batch fermentation

For aerobic cultivation of yeast the cell mass concentration, Xt, and the concentration of the limiting substrate component (glucose), St, has been determined.

Time (min)

0

30

60

90

120

150

180

210

240

Xt (gDCM*/l)

5.0

5.2

6.6

8.4

10.8

13.8

17.7

19.0

20.0

St ( g glu/l)

30.0

29.6

26.8

23.2

18.4

12.4

4.6

2.0

0.0

*Dry Cell Mass

a. Calculate the average cell mass yield factor, YX/S in this fermentation.

b. Calculate the maximum specific growth rate, mMAX, in the logarithmic growth phase.

c. Calculate the volumetric growth rate, dX/dt = rX at time=125 min.

d. Calculate the volumetric substrate utilization rate, rS, and the specific substrate utilization rate, qS, of the yeast at time = 170 min, assuming that the average yield factor can be used to determine rS.

e.Calculate the concentration of left over substrate at time = 130, again assuming that the average yield factor can be used.

Posted Date: 3/21/2013 3:07:16 AM | Location : United States







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