Calculate the enzyme concentration in the reaction mix, Physics

For an enzyme that follows Michealis-Menten kinetics, the initial velocity at different substrate concentration has been determined.

[S] (mM)

0.1

0.3

1.0

3.0

10.0

30.0

v0 (mM/min)

0.21

0.50

0.94

1.25

1.42

1.47

a. Determine KM and VMAX

b. The experiments are replicated with addition of a reversible inhibitor (the new results are shown in below table). Determine the effect of the inhibitor on KM and VMAX and the type of inhibition.

[S] (mM)

0.1

0.3

1.0

3.0

10.0

30.0

v0 (mM/min)

0.12

0.30

0.68

1.07

1.34

1.44

c. Calculate the enzyme concentration in the reaction mix, when the turnover number of the enzyme, kcat is 250 s-1.

 

Posted Date: 3/21/2013 2:58:54 AM | Location : United States







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