For an enzyme that follows Michealis-Menten kinetics, the initial velocity at different substrate concentration has been determined.
a. Determine KM and VMAX
b. The experiments are replicated with addition of a reversible inhibitor (the new results are shown in below table). Determine the effect of the inhibitor on KM and VMAX and the type of inhibition.
c. Calculate the enzyme concentration in the reaction mix, when the turnover number of the enzyme, kcat is 250 s-1.