Calculate the enzyme concentration in the reaction mix, Physics

For an enzyme that follows Michealis-Menten kinetics, the initial velocity at different substrate concentration has been determined.

[S] (mM)

0.1

0.3

1.0

3.0

10.0

30.0

v0 (mM/min)

0.21

0.50

0.94

1.25

1.42

1.47

a. Determine KM and VMAX

b. The experiments are replicated with addition of a reversible inhibitor (the new results are shown in below table). Determine the effect of the inhibitor on KM and VMAX and the type of inhibition.

[S] (mM)

0.1

0.3

1.0

3.0

10.0

30.0

v0 (mM/min)

0.12

0.30

0.68

1.07

1.34

1.44

c. Calculate the enzyme concentration in the reaction mix, when the turnover number of the enzyme, kcat is 250 s-1.

 

Posted Date: 3/21/2013 2:58:54 AM | Location : United States







Related Discussions:- Calculate the enzyme concentration in the reaction mix, Assignment Help, Ask Question on Calculate the enzyme concentration in the reaction mix, Get Answer, Expert's Help, Calculate the enzyme concentration in the reaction mix Discussions

Write discussion on Calculate the enzyme concentration in the reaction mix
Your posts are moderated
Related Questions
Wien's displacement law constant, b It is the constant of the Wien displacement law. It contains the value 2.897 756 x 10 -3 m K.


An iron ring of mean diameter 10 cm is uniformly wound with 2000 turns of wire. When a current of 0.25 A is passed by the coil a flux density of 0.4 T is set up in the iron. F

Im having trouble understanding these,, could someone explain them in lamens terms

write principles,construction & working of a moving coil galvanometer

For the double-beam oscilloscope displays shown in Figure, determine (a) their frequency (b) their r.m.s. values (c) their phase difference. The 'time/cm' switch is on 10

A pith ball weighing 3.0 x 10 -2 N carries a charge of -1.0 x 10 -6 C. The pith ball is placed among two large, parallel, metal plates that are divided by 0.050 m. What is

Alnico is a family of alloys containing metals Fe, Co and Ni plus small amount of Al and copper. These alloys are directionally solidified and subsequently given a heat treatment t

Can a solid and liquid together be possible? Actually, substances can exist in all three states of matter concurrently if the conditions are right. The triple point of a substa

Explain about the double-crucible method. Low loss fibers are mostly manufactured by using double crucible and vapour phase deposition method. The double-crucible method is