Calculate the amount of glycine in the mixture, Chemistry

Calculate the amount of glycine in the mixture:

Isotope dilution analysis was employed for the determination of glycine in 5.83 g protein hydrolysate mixture by using 14C labelled compound. 5.3 mg labeled compound with an activity of 39856 cpm was added to the mixture. The counter had background counts of 11790 counts in 90 min. After thorough mixing, the solution was passed through an ion-exchange column when 3.7 mg pure glycine was separated which showed an activity of 10900 counts in 50 min using the same counter. Calculate the % amount of glycine in the mixture.

Answer:

The counter had a background of 11790 counts in 90 min

or Background count rate =  131 cpm

Amount of radiolabeled tracer added, m*           =          5.3 mg

Specific Activity of the radiotracer added, SA1   = (39856 -131)/25.7

= 39725/25.7

=   1545.7 cpm/mg

Amount of pure fraction of glycine = 23.7 mg

Net Activity of glycine = 10900 counts in 50 min = 218 cpm - 131 cpm(background)

= 87 cpm

Specific Activity of separated fraction of glycine = 87 / 3.7 = 23.5 cpm/mg

Amount of glycine in mixture = 5.3 (1545.7/23.5 - 1) = 343.1 mg

Percent amount of glycine in the mixture = (0.343/5.83) ×100

= 5.89%

Posted Date: 1/2/2013 6:36:32 AM | Location : United States







Related Discussions:- Calculate the amount of glycine in the mixture, Assignment Help, Ask Question on Calculate the amount of glycine in the mixture, Get Answer, Expert's Help, Calculate the amount of glycine in the mixture Discussions

Write discussion on Calculate the amount of glycine in the mixture
Your posts are moderated
Related Questions
how can you explain the fact that lanthinides are highly electopositive?


o-isomer is more volatile and how separated by steam distillation?

Why do Zr and Hf show similar properties?


Describe Hall Effect? Briefly discuss physical origin and uses of Hall Effect? Ans: If a conducting bar is placed in a magnetic field B ⊥ to its axis and if a current flow

perkin ring closure reaction of melonic ester with C2H5O-Na+

give an example of a compound whose empirical formula & molecular formula are same.

what is the definition of glucogenesis?

Q. Explain scale formation and sludge formation in boilers. How are they formed?     Ans Boilers are used for steam generation. When hard water is evaporated, progressively