Beta catenin for nuclear import, Science

1.  Beta catenin is a protein that translocates to the nucleus in the presence of Wnt ligand. In the absence of Wnt, beta catenin is targeted for degradation. Using a cell line from a new species of frog, Xenopus simulatus, you have found a protein that you believe is homologous to beta catenin, as it translocate to the nucleus in the presence of Wnt, but does not appear to be degraded in the absence of Wnt. You call this protein Better catenin.

 You're interested in determining which regions of  Better catenin are responsible for its localization within the cell. You generate deletion mutants of each of Better catenin's 5 regions (shown below), all fused to GFP.   You express each construct in your cell line and examine the localization of Better catenin in the presence and absence of Wnt (Table 1).  In both charts below, Δ stands for "deletion", so only the ΔX portion of the protein is missing.  

1700_Beta Catenin for Nuclear Import.png

A) Which region of Better catenin is necessary for nuclear import, and what does this region contain?

B) In a second experiment, you express your deletion mutants in cells that are exposed to Wnt. After 30 minutes you wash Wnt away from the cells and observe the cellular localization of Better catenin. Your results are summarized in Table 2. What is your interpretation of these results?

C) Finally, using your wild-type GFP-tagged protein, you examine Better catenin localization in a cell line that has a non-functional Ran-GEF. Predict what would happen to Better catenin in the absence and presence of Wnt, and when Wnt is washed away as before, and describe where you would see GFP.

Posted Date: 3/6/2013 5:39:38 AM | Location : United States







Related Discussions:- Beta catenin for nuclear import, Assignment Help, Ask Question on Beta catenin for nuclear import, Get Answer, Expert's Help, Beta catenin for nuclear import Discussions

Write discussion on Beta catenin for nuclear import
Your posts are moderated
Related Questions
what is the weigt on earth of an object that has a mass of 22 kg?

physical features of arizona

how does fault block mountains affect people in that area

How to make an experimental biter Fit a one-hole stopper carrying a short length of glass tube into the small end of a lamp chimney. Put a little cotton wool in the bottom and

Dating  the Past: Today, that  a particular  rock  is a million ycars old, or a fossil is 50 thousand ycars old. For  instance, how do we estimate the duration of various geo

Kwashiorkar: This disease was first recognised in  1935,  in African children and it  is named from two words of an African dialect, meaning first and second, i.e.  sickness th

Define the Lymphocytes They are agranulocytes. The nucleus occupies approximately the entire cell. They vary in size from 6- 18 mm diameter; sometimes divide in small and large

#2 DESCRIBE THE APPEARANCE OF THE STOMATE AND THE GUARD CELLS.

Explain Rh Incompatibility Rh compatibility sometimes leads to complications which are generally not seen with other types of mismatched transfusions. If a Rh negative is given

What is full form of WHO ?