Autoradiography, Biology

Autoradiography is the process to detect radioactively labeled molecules (which commonly have been separated in an SDS-PAGE or agarose gel) based on their ability to develop an image on photographic or X-ray film. This procedure does not result in a linear relationship between the intensity of the signal and the amount of the radioactivity unless special steps are taken. There is now increasing use of the phosphorimagers and other modern devices to detect and quantitative radioactive molecules which have been separated in gels.

Posted Date: 8/1/2012 7:09:43 AM | Location : United States







Related Discussions:- Autoradiography, Assignment Help, Ask Question on Autoradiography, Get Answer, Expert's Help, Autoradiography Discussions

Write discussion on Autoradiography
Your posts are moderated
Related Questions
Q. Serotonin - Enhancement of memory? Increased level of serotonin is implicated in the enhancement of memory. It plays a significant role in classical conditioning wherein sti

Given how the Na+/K+ pump works, which part of the cell (inside or outside) will have an excess of negative charge?

Digestion of Nucleic Acids Several nucleases present  in  the  pancreatic juice  like RNAase and  DNAase  digest various nucleic acids- These enzymes  are endonucleases which h


Terrestrial and Aquatic Ecosystem What is common between a terrestrial and an aquatic ecosystem. Both have similar type of trophic structure. They possess an autotrophic layer

Q. What are the catalysts? Catalysts are substances that decrease the activation energy of a chemical reaction, facilitating it or making it energetically viable. The catalyst

Anomalies Related to Oesophagus and Stomach   Let us discuss briefly the anomalies of oesophagus and stomach. These are summarised as  follows: Associated Problems  1)  P

Explain the Pregnancy and Obesity? Obesity is associated with increased risk for gestational diabetes, hypertension, pre- eclampsia, perinatal mortality and the need for induce

Define Difference between Plant Systematic and Taxonomy? Before coming to principles of taxonomy let us discuss the difference between taxonomy and systematic. Systematic is th

MR means medical representative. Medical rep has to sell products of pharma companies or drug manufacturers. He has to go to doctors and medical shops to sell drugs and promote dru