Atomic fluorescence spectrometry, Chemistry

Assignment Help:

Atomic fluorescence spectrometry:

In atomic fluorescence spectrometry, the gaseous atoms acquired through flame or electrothermal atomisation are excited to higher energy levels through absorption of the electromagnetic radiation and the fluorescence emission from these excited atoms is measured. A fluorescence emission could occur by different pathways. Therefore, we have various types of atomic fluorescence transitions. The general types of atomic fluorescence transitions are termed as resonance fluorescence, Stokes direct line fluorescence, stepwise line fluorescence, two step excitation or double resonance fluorescence, sensitized fluorescence and thermal fluorescence. Of these, the thermally assisted fluorescence and sensitized fluorescence commonly are not employed for analytical reasons.

The intensity of the fluorescence radiation is measured at right angles to the direction of incident radiation and is associated to the concentration of the element present, creating the basis of quantitative analysis. In quantitative atomic fluorescence spectrometric determinations the instrument is commonly standardised through a calibration curve. The graph is drawn among the logarithms of the intensity of atomic fluorescence signal versus the log of analyte concentration. The linearity of like curves extends over 3 to 5 sequences of magnitudes. Thus, at higher concentration, linearity is lost because of self absorption.


Related Discussions:- Atomic fluorescence spectrometry

Write the maximum possible coordination number of atom, Write the maximum p...

Write the maximum possible coordination number of an atom in an hcp crystal structure of an element? Illustrate the formula relating pressure of a gas with its mole fraction in

#space lattice, #space lattice, unit cell, calculation of particles per uni...

#space lattice, unit cell, calculation of particles per unit cell, laws of crystallography, symmetry elements in crystals, derivation of braggs equation#

Functional group tests, how do we test for functional groups in chemistry l...

how do we test for functional groups in chemistry lab

Solutions, how to determine the percentage of association or dissociation i...

how to determine the percentage of association or dissociation in a solution?

Uses of proteins - biomolecules, Uses of Proteins (a) Proteins constitu...

Uses of Proteins (a) Proteins constitute as necessary part of our food. Meat, eggs, fish, cheese offer proteins to human beings. (b) In textile: Casein (a milk protein) is u

Describe hall effect and uses of hall effect, Describe Hall Effect? Briefly...

Describe Hall Effect? Briefly discuss physical origin and uses of Hall Effect? Ans: If a conducting bar is placed in a magnetic field B ⊥ to its axis and if a current flow

Find the pressure in the droplet, Q. The pressure in a liquid droplet of ra...

Q. The pressure in a liquid droplet of radius r is greater than the pressure of the surrounding equilibrated gas phase by a quantity 2 =r, where is the surface tension. (a) Cons

Uses of phenol - hydrocarbon, Uses of Phenol - Hydrocarbon Phenol is ex...

Uses of Phenol - Hydrocarbon Phenol is extensively used in industry. The important applications of phenol are (i) Phenol is used as an antiseptic in soaps, lotions and ointm

Acid base titration, the given solution contains 2.5g of laundry soap disso...

the given solution contains 2.5g of laundry soap dissolved per 250 cubic cm.determine the amount of free alkali in 350 cubic cm.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd