Atomic fluorescence spectrometry, Chemistry

Atomic fluorescence spectrometry:

In atomic fluorescence spectrometry, the gaseous atoms acquired through flame or electrothermal atomisation are excited to higher energy levels through absorption of the electromagnetic radiation and the fluorescence emission from these excited atoms is measured. A fluorescence emission could occur by different pathways. Therefore, we have various types of atomic fluorescence transitions. The general types of atomic fluorescence transitions are termed as resonance fluorescence, Stokes direct line fluorescence, stepwise line fluorescence, two step excitation or double resonance fluorescence, sensitized fluorescence and thermal fluorescence. Of these, the thermally assisted fluorescence and sensitized fluorescence commonly are not employed for analytical reasons.

The intensity of the fluorescence radiation is measured at right angles to the direction of incident radiation and is associated to the concentration of the element present, creating the basis of quantitative analysis. In quantitative atomic fluorescence spectrometric determinations the instrument is commonly standardised through a calibration curve. The graph is drawn among the logarithms of the intensity of atomic fluorescence signal versus the log of analyte concentration. The linearity of like curves extends over 3 to 5 sequences of magnitudes. Thus, at higher concentration, linearity is lost because of self absorption.

Posted Date: 1/10/2013 2:37:44 AM | Location : United States

Related Discussions:- Atomic fluorescence spectrometry, Assignment Help, Ask Question on Atomic fluorescence spectrometry, Get Answer, Expert's Help, Atomic fluorescence spectrometry Discussions

Write discussion on Atomic fluorescence spectrometry
Your posts are moderated
Related Questions
Q. What do you mean by sensory perception? The appearance of a food can be evaluated in terms of colour, surface characteristics such as smoothness of a surface, dry surface, g


Q. What are Native Minerals? These minerals contain the metal in free or elemental state, e.g., copper, silver, gold, platinum and iron. The metals are usually found mixed with

Explain Modelling for process and recipe Generally, all problems that appear in food product design can be divided into mixture or process problems, with the latter having th

The de-Broglie wavelength associated with a particle of mass  10 -6 kg moving with a velocity of 10 ms -1 , is: (1) 6.63 x 10 -22 m                (2) 6.63 x 10 -29 m

how to prepare westron from acetylene

Rutherford's alpha particle scattering experiment eventually led to the conclusion that: (1)The point of impact with matter can be precisely determined (2)Electrons occupy s

Q. How to produce Nitrogen on Industrial Level? Ans. Nitrogen is produced by the fractional distillation of liquid air. Air is cooled to liquefy it. The liquid is then all

Q. Application of electrometallurgy? In theory, aluminium metal would be made the same way. But, aluminium trichloride is covalent and it does not conductor of electricity. As

Q. Define Egg foam stability? The two factors of utmost importance in egg white foams are stability and volume. Several factors influence one or both of these characteristics