Atomic fluorescence spectrometry, Chemistry

Atomic fluorescence spectrometry:

In atomic fluorescence spectrometry, the gaseous atoms acquired through flame or electrothermal atomisation are excited to higher energy levels through absorption of the electromagnetic radiation and the fluorescence emission from these excited atoms is measured. A fluorescence emission could occur by different pathways. Therefore, we have various types of atomic fluorescence transitions. The general types of atomic fluorescence transitions are termed as resonance fluorescence, Stokes direct line fluorescence, stepwise line fluorescence, two step excitation or double resonance fluorescence, sensitized fluorescence and thermal fluorescence. Of these, the thermally assisted fluorescence and sensitized fluorescence commonly are not employed for analytical reasons.

The intensity of the fluorescence radiation is measured at right angles to the direction of incident radiation and is associated to the concentration of the element present, creating the basis of quantitative analysis. In quantitative atomic fluorescence spectrometric determinations the instrument is commonly standardised through a calibration curve. The graph is drawn among the logarithms of the intensity of atomic fluorescence signal versus the log of analyte concentration. The linearity of like curves extends over 3 to 5 sequences of magnitudes. Thus, at higher concentration, linearity is lost because of self absorption.

Posted Date: 1/10/2013 2:37:44 AM | Location : United States







Related Discussions:- Atomic fluorescence spectrometry, Assignment Help, Ask Question on Atomic fluorescence spectrometry, Get Answer, Expert's Help, Atomic fluorescence spectrometry Discussions

Write discussion on Atomic fluorescence spectrometry
Your posts are moderated
Related Questions
The intramolecular product tetrahydrofuran results from an internal nucleophilic substitution reaction of the alkoxide on the alkyl halide. The intermolecular product 1,4-butanedio

When 100 ml of M/10 H2SO4 is mixed with 500 ml of My/10 NaOH then nature of resulting solution and normality of excess reactant left is

Which of the following is ideal for Oxidation of propene to propan-1-ol a)Alkaline KMnO 4 b)B 2 H 6 /Alkaline KMnO 4 c)O 3 /Zn dust d)CrO 3   Ans) B 2 H 6 /Alkaline KMno4 is th


Valence electron of pd Pd has ten valence electrons in the neutral atom.   There are no charges and no X-type ligands in the complex; as, this is a d 10 complex.

does lowered pH of water affect dissolved oxygen?

Q. Test for hydrocyanic acid? Hydrocyanic acid is present as an impurity in synthetic ethyl isothiocyanate, which is employed to make the flavour of mustard oil more pungent. T

Chemical Properties Proteins - Hydrolysis Hydrolysis : The very simple proteins are hydrolysed by acids, alkalies or enzymes to generate amino acids. Subsequent steps are inclu


Write a sort note electronic displacements in a covalent bond ?