Artificial skin, Biology

Artificial Skin:

The skin generated in vitro is in fact the epidermis portion of skin only; when the epidermis is applied to the burnt part, this leads to the regeneration of dermis (remaining parts of skin) beneath. But improvements in the approach  have allowed the reconstitution of virtually complete skin (both epidermis and dermis), called as living skin equivalent (LSE); this technology uses a collagen matrix as a support for development of the tissue. The skin explants used for attaining artificial skin may be either obtained from the patient concerned or from the foreskin (loose skin from the tip of penis) of newborn. Skin cells of new-borns grow more strongly than adult skin; the use of a synthetic polymer called as PGA helps the newborn skin to develop with no scars. Artificial skin from newborn skin explants is used to wrap the wound until the patient's skin is cultured and artificial skin is gained for grafting.

The production of artificial skin is following   and this is particularly cell and not organ culture. The bulk (Ca 90%) of epidermis is constituted by cells that called keratinocytes which generated the dead cells (corneocytes) making up the outermost cornfield layers of skin. The keratinocytes are dissociated by treating the skin explants with trypsin. These cells are sophisticated in vessels the bottom of which is enclosed with irradiated 3T3 fibroblast cell line; this is because certain products from fibroblast cells facilitate the proliferation of keratinocytes. Keratinocytes develop to make colonies, which are again dissociated into single cells and cultured in the same manner. The process is repeated again and again till a confluent sheet of pure epithelium is completed; this sheet is detached from the culture vessels, cleaned and used for grafting. The explants for developing artificial skin for the graft might come from the patient himself to avoid rejection. A 3 cm2 skin explants may yield about 1.7 m2 artificial skin in 3-4 weeks representing a 5000-fold increase. In approximate 5 years after grafting of the artificial skin, all the essential components of skin are regenerated. Artificial skin grafts have been used to successfully repair several types of skin defects, including chronic skin ulcers.

Posted Date: 10/2/2012 1:21:36 AM | Location : United States







Related Discussions:- Artificial skin, Assignment Help, Ask Question on Artificial skin, Get Answer, Expert's Help, Artificial skin Discussions

Write discussion on Artificial skin
Your posts are moderated
Related Questions
A Molecule of DNA is 2.17 um long. The ends of the molecule become strongly ionixed: negative on one end, positive on the other. The helical molecule acts like a spring and compres

In a plasma membrane, which of the below provides a general barrier to the movement of molecules? a) Lipids b) Proteins c) Carbohydrates d) all of these Answer: a

Initiation Codon is the codon at which the translation of a polypeptide chain is initiated. This is generally the first AUG triplet in the mRNA molecule from 5' end, where the rib

Q What is the main source of energy for living beings on earth? The sun, center of our planetary system and star of the Milky Way galaxy (our galaxy), is the source of the ener

In the following pedigrees, the disorders or traits presented follow simple patterns of Mendelian inheritance. For each trait, determine the most probable mode of inheritance, stat

How does the embryo turn from gastrula into neurula? How is the neural tube formed? What is the embryonic origin of the nervous system in vertebrates? The neurula stage is divi

What is Nucleotides? Nucleotides are the basic building blocks of nucleic acids. Each nucleotide is composed of a nitrogen base, a 5-carbon sugar called a pentose, and an ass

Science as a human endeavour: Science is a human endeavour. Human beings, from prehistoric times, attempted to control nature for their own welfare. For this, they had to ob

Define Tests of Cardiovascular Endurance? Cardiovascular endurance or aerobic fitness is the ability to exercise continuously for extended periods without tiring. It can be me

Why do we need a family of proteins for cell adhesion (what is the advantage of having a family of proteins?)