Antibodies, Biology

Antibodies

Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect

In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined under
a microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.

618_antibodies.jpg

Posted Date: 12/10/2012 1:18:12 AM | Location : United States







Related Discussions:- Antibodies, Assignment Help, Ask Question on Antibodies, Get Answer, Expert's Help, Antibodies Discussions

Write discussion on Antibodies
Your posts are moderated
Related Questions
Where would a predicted silent mutation have to be situated to actually result in a loss-of-function mutation (and potentially lead to the onset of disease)? A. Intron-exon jun

Tricuspid Annuloplasty :  Tricuspid valve is evaluated by digital palpation before connecting the patient to cardio pulmonary by pass. Functional regurgitation can be repaire

Why can mitochondria be considered the power plants of the aerobic cells? Ans) Mitochondria are the "power plants" of aerobic cells due to within them the final stages of the ce

Explain Ventricular Septal Defect in details ? Indications of surgery :  Some VSDs close spontaneously or become smaller in size. This has to be taken into consideratio

Define the terms umbra and penumbra, and describe how they relate to solar eclipses.

nervous system of vertebrates

What is an etiological agent of disease? An etiological agent of disease is the agent that causes the disease. It might be a living being, substance or environmental fact.

Explain Food Applications of pullulan The food applications of pullulan include:  Due to its oxygen barrier properties, it can be used as a packaging material for fat /

Chemical Stress - Atmospheric imbalances At least two components of the environment, oxygen and carbon dioxide that plants require are predominantly in the gaseous form. As yo

Q. What is the nitrogen cycle? The nitrogen cycle represents the recycling and circulation of the chemical element nitrogen in nature. The nitrogen cycle fundamentally depends