Analysis of metabolic components , Chemistry

1. Produce a figure with an appropriate figure legend of your SDS-PAGE gel that could be published in a scientific journal.

  •   Indicate all relevant information, e.g.origin, front, size of the molecular mass markers and casein and whey protein bands. You can use e.g. Power-Point, Photoshop or Illustrator or similar software for his task
  •   Write a concise figure legend with a minimal number of words that allows the reader to understand what he sees. Note: Give some information about the methods but do not repeat all details in the legend!

2. Fill in the table to report your calculated estimates for the molecular masses (kDa) of the proteins in the milk sample. You can enter up to 3 different significant bands that you observed in the respective fractions.

Protein fraction

Estimated molecular mass (kDa)

Casein proteins

 

 

 

Whey proteins

 

 

 

Small molecular mass proteins

 

 

 

3. Draw a graph of absorbance against fraction number for the gel filtration chromatography. Using Excel you will need to create a graph with two axes (one axis on the left for the absorbance of haemoglobin (Abs 400 nm) and one axis on the right for the absorbance of vitamin B12 (Abs 550 nm).

4. Explain in molecular terms the observed movement of the various sized proteins through:

a) the polyacrylamide gel

b) the gel filtration column

5. A cell consists of thousands of individual molecular components. To understand biochemical pathways is often necessary to separate, purify and analyse the molecular constituents of a cell. Name three different biochemical/biophysical properties in which biological molecules differ from each other and think about a corresponding technique that can be exploited to separate and/orcharacterize such metabolites.

Biochemical/Biophysical property

Technique that exploits this property

Example given: different ionic charge

Example given: separation on gel in electric field

 

 

 

 

 

 

6. Consider an individual who had to dash for 500 metres in order to catch a tram that was just about to depart. Indicate thelikely concentration of metabolites that molecular analysis would reveal in the muscle tissue of this person. Increased, decreased and unchanged are describing levels relative to a healthy resting state.

Metabolite

Concentration

(Increased, decreased or unchanged)

acetyl-CoA

 

pyruvate

 

lactate

 

glucose (blood)

 

glucose-6-phosphate

 

EtOH

 

glyceraldehyde-3-phosphate

 

AMP

 

glucose-1-phosphate

 

ephinephrine

 

Posted Date: 2/16/2013 7:28:03 AM | Location : United States







Related Discussions:- Analysis of metabolic components , Assignment Help, Ask Question on Analysis of metabolic components , Get Answer, Expert's Help, Analysis of metabolic components Discussions

Write discussion on Analysis of metabolic components
Your posts are moderated
Related Questions
A nucleophile is a chemical species that donates an electron pair to an electrophile to form a chemical bond in a reaction. All molecules or ions with a free pair of electrons or a

How do you mean by chemistry?


The number of 2p  electrons having spin quantum numbers= -1/2   are: (1) 6        (2) 0        (3) 2        (4) 3 Ans: 3


Q. What are Native Minerals? These minerals contain the metal in free or elemental state, e.g., copper, silver, gold, platinum and iron. The metals are usually found mixed with

What is the principle of determining acid insoluble ash content of flour

The oxygen of the hydronium ion The oxygen of the hydronium ion has approximately tetrahedral geometry and is therefore sp 3- hybridized. Two of the sp 3 hybrid orbitals conta

Crude oil is separated into several fractions, each of these fractions are composed of many undefined hydrocarbon components. For simplicity, the crude oil is assumed to be a mixtu

We performed a titration in the lab with mock gastric juice and potassium hydroxide. We titrated to end point and then to another colour change and noted both the volumes. We are n