Agarose gel electrophoresis, Biology

Agarose gel electrophoresis is the method to analyze the size of the DNA (or RNA) fragments. In the presence of an electric field, bigger fragments of DNA move through a gel slower than the smaller ones, producing different migrating "bands". Generally, these are visualized by soaking the gel in a dye (ethidium bromide) which makes the DNA fluoresce under UV light. This is the gel of choice for the DNA or RNA in the range of thousands of bases in length, or up to 1 megabase if you are using pulsed field gel electrophoresis. 

Posted Date: 8/1/2012 7:02:28 AM | Location : United States







Related Discussions:- Agarose gel electrophoresis, Assignment Help, Ask Question on Agarose gel electrophoresis, Get Answer, Expert's Help, Agarose gel electrophoresis Discussions

Write discussion on Agarose gel electrophoresis
Your posts are moderated
Related Questions
Minerals :- Magnesium    Food Source      Whole grains, nuts, legumes, green leafy vegetables Nutritional Functional role Essential nutrient: Deficiency is rare Co

Muromonab CD-3 (Orthoclone, OKT-3) A monoclonal antibody against mature T lymphocytes, OKT-3 is primarily used to treat acute rejection episode. IV for 10-14 days. Effe

What are the two reactants and the two products of a dehydration reaction/ what are the two reactants and products of a hydrolysis reaction.

Natural versus Artificial (Man-made) Environment The environments discussed so far are, natural environments. In several instances man has greatly altered the natural condition

polymorphism and its causes

Q. What is the official name of pyruvic acid? Pyruvic acid is named as 2-oxopropanoic acid it is thus a molecule made of three linearly bound carbons with one extremity forming

Circulatory Assist Devices :  Intra aortic balloon pump (IAUP) was introduced by Kantrowitz (19hX). It is also known as Counter pulsation or Diastolic augmentation.

Please consider the phenomenon of bacterial resistance to antibiotics. How did the combination of human advances and basic evolutionary principles lead to this situation? How is it

Q. How are gametes formed in the pteridophyte life cycle, by mitosis or meiosis? What is the type of meiosis that occurs in pteridophytes? In pteridophytes gametes are made by

Which part of the carbon cycle is responsible for the enhancing concentration of carbon dioxide in the atmosphere. The burning of fossilized products (coal and oil) of some pla