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1. Why does Synechocystis not require electroporation or chemical pretreatment prior to transformation?2. What specific genetic manipulation does Synechocystis readily allow that E. coli does not?3. For transformation of Synechocystis, why do you first plate your transformant mixture on a filter on a BG - 11 plate with glucose, and the next day the TA transfers the filter with cells to a plate that contains kanamycin (rather than plating directly on kanamycin)?4. What is the product of the psbC gene? What is its function?5. In the PCR reaction, what is the role of each of the PCR reagents: primers, dNTPs, Mg2+, and Taq polymerase?6. What are the considerations in determining the number of cycles needed for the PCR reaction?
What is the difference between decontamination, sterilization, sanitization, and degermation? Which one offers the highest level of control? Which one offers the lowest level of control?
Her breath smells like acetone and her blood glucose test out at 650mg/100ml of blood. She is in acidosis. What hormone must be administered to her and why. Where may she find such DNA.
A white albino mouse was crossed with a wild grey colored one. The first generation mice were all grey. In the second generation, there were 198 grey and 72 albino mice.
List in chronological order five major discoveries or developments in molecular biology and bioinformatics since the early 1950's, which you consider responsible for the current state of these fields. Provide a brief explanation for each of your c..
Normal cells greatly slow their rates of cell division after filling a culture dish with a layer that is one cell deep. If a circular group of cells is removed from the middle of the filled culture dish,
With regard to the 5' - 3' orientation of the nontranscribed DNA strand, is the order of the genes AB or BA?
Both endocrine and nervous system are major regulating system of the body; but nervous system has been compared to an airmail delivery system and the endocrine to pony express. Briefly explain the comparison.
73.7 mL of stock ethanol was added to 26.3 mL of distilled H20 to generate a 70% ethanol solution. What was the concentration of the initial stock ethanol?
Complex, Simple and Hypercomplex (Endstop cells) cells. Layer 4C and synapse onto simple cells. Whether or not they aer bars of light Simple cell is listening to a group of LGN cells. So each increment of 2.5 degrees has different simple cells.
What factors establish the choice of a paper strip containing bacterial endospores or a sealed ampule containing an endospore suspension for testing heat-sterilization equipment.
What are the roles of the cell surface, the cytoskeleton and intercellular signaling in development? Include examples from gastrulation, induction, morphogenesis and axis formation.
In fruit flies (Drosophilia melanogaster), Apterous (wingless) is an autosomal recessive trait to the wild type, normal wings. White eyes in Drosophilia is X linked and recessive to the wild type, red eyes.
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