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Consider the following reactions, which are two consecutive steps in the TCA cycle.
The two reactions are catalyzed by two independent enzymes, malate + NAD + ↔ oxaloacetate + NADH ΔG10 = +29.7 kJ/mol acetyl-CoA + oxaloacetate ↔ citrate + CoA ΔG10 = -31.5 kJ/mol.
How can the first reaction in the list proceed in the direction of products in this pathway with such a high positive free energy change?
a. this reaction is coupled to ATP hydrolysisb. the second reaction isn the list uses the oxaloacetate keeping its concentration low resulting in a ΔG that is about zero for the first reaction.c. the Q for the first reaction in the list is much larger than its Keq and as such is irreversibled. the answer to this question cannot be determined from the information given
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