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The interaction between bases on different strands of DNA molecules depends somewhat on the identities of neighboring bases. For example, the melting temperature of an oligonucleotide with the sequence GGGGGAAAAA is different than that of GAGAGAGAGA even though both contain the exact same number of G-C and A-T base pairs. (The melting temperature is the temperature at which half of the base pairs are broken between the oligonucleotide and its complementary sequence). Therefore, instead of treating a DNA helix as a string of interactions between base pairs, many thermodynamic algorithms that calculate DNA melting temperatures use the nearest-neighbor model, which treats a DNA helix as a string of interactions between 'neighboring' base pairs. Thus the sequence GGCTGC consists of the nearest neighbors GG, GC, CT, TG and GC. The nearest-neighbor frequencies of a certain double stranded DNA molecule are: AG, 0.15; GT, 0.03; CC, 0.08, and TT, 0.10. In each case the nearest neighbor is written in the 5'?3' direction. What would be the nearest neighbor frequencies of CT, AC, GG and AA?
Explain how you would prepare 200 ML of a 0.25 Msoluation of NaOH from a 3 M stock solution?
So far while surgery was performed on his neck, the surgeon might not find the parathyroid glands at all. Where should the surgeon look next to find out the tumorous parathyroid gland.
You are a neuroengineer interested in developing better nerve stimulators for prothetic limbs, such as arms, hands or legs. You want to understnad the flow of ions across the typical nerve cell membrane during action potentials, or spikes.
What is the significance, while setting threshold for adverse effects, of the fact that, broadly speaking, solid particulates have much longer biological half times than gases and vapours.
Mice are diploid with 2n=40. Determine how many chromosomes make up the genome and how many chromosomes would you expect to see in a mouse gamete.
The pH of the inner thylakoid space had been measured, as have the pH of the stroma and the cytosol of a particular plant cell. Why will this cause the pH in the thylakoids to be lesser than the pH in the stroma.
Three bases per codon are the minimum necessary to code for twenty different amino acids when four different bases are involved.
Discuss why DNA is the most favorable molecule for genetic material and how RNA compares to it in this respect?
An alien spacecraft has crash landed on Ram Plaza here on campus. One of the space travelers seems to have survived, but with very serious injuries.
Utilize your imagination to describe two ways in which disturbance of the sort just described may affect the animals that live on the panels and amount of free space available.
Histones are proteins found in the nucleus tightly bound to DNA, Determine which has many phosphate groups. The pl of histones is very high, about 10.8.
Contained is a description of the signaling cascade that results from EGF stimulation of the Ras-MAPK pathway in cells.
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