What will a graph of the effect of ph on the activity

Assignment Help Biology
Reference no: EM13326614

Suppose that an enzyme uses a catalytic mechanism in which a functional group on the enzyme has to accept a proton from the intermediate formed in the first chemical step. The pKa of that basic functional group on the enzyme is 8.0, and is the only pKa apparent on a plot of kcat vs pH for the activity of this enzyme. The kcat of the fully active form of the enzyme is 1 X 10 ^5 sec^-1.

1. What will a graph of the effect of pH on the activity of this enzyme look like? (Graph of kcat vs pH)

2. What type of functional group might be responsible for the pH effect on kcat? Justify your anser based on the generic pKa values of functional groups in peptides and proteins..

Reference no: EM13326614

Questions Cloud

What is the separation between the plates : A parallel-plate air capacitor is to store charge of magnitude 290pC on each plate when the potential difference between the plates is 36.0V, If the area of each plate is 6.80 cm2, what is the separation between the plates
Compare and contrast the codes adopted by the ICIFI : Compare and contrast the codes adopted by the ICIFI and by Abbott Laboratories. Assess the codes in terms of utilitarian criteria, in terms of their recognition of moral rights, and in terms of their adherence to standards of justice. What explanatio..
Calculate the area of each plate : The plates of a parallel-plate capacitor are 3.00mm apart, and each carries a charge of magnitude 75.0nC. What is the area of each plate
Obtain the magnitude of the centripetal force : In a skating stunt known as crack-the-whip, a number of skaters hold hands and form a straight line. obtain the magnitude of the centripetal force
What will a graph of the effect of ph on the activity : The pKa of that basic functional group on the enzyme is 8.0, and is the only pKa apparent on a plot of kcat vs pH for the activity of this enzyme. The kcat of the fully active form of the enzyme is 1 X 10 ^5 sec^-1.
Calculate the speed of the stone : A 0.60 kg stone attached to a string is whirled in a horizontal circle of radius 43 cm as in the figure below. The string makes an angle of 35° with the vertical. Find the speed of the stone
What is the frequency of the em wave : In SI units, the electric field in an electromagnetic wave is described by Ey = 100 sin(107x - ?t ). What is the frequency of the EM wave
Understand self-government to mean democracy : "The United States is often described as an experiment in self-government. Most people understand "self-government" to mean democracy. According to this understanding, people are self-governed if they regularly vote to select the individuals who are ..
What is the frequency of these microwaves : A radar unit sends out 0.05 microsecond pulses of microwaves whose wavelength is 25 mm. What is the frequency of these microwaves

Reviews

Write a Review

 

Biology Questions & Answers

  Write important properties of water and phospholipids

BIO328- Biology for engineers, Assignment I: -  Write important properties of water and phospholipids due to which life exists on the earth. If double stranded DNA has 14% G (guanine), what percent A (adenine), T (thyamine) and C (cytosine) would yo..

  Organisms recognize others as members of their own species

Brown eyes (B) are dominant over blue eyes (b) in humans. If a brown-eyed man marries a blue eyed woman and they have three kids, and two of the three have brown eyes and other has blue, what would be the punnett square indicating so look like.

  Which types of energy can be transformed into heat

Which of the following types of energy can be transformed into heat?

  Contrast a hypothesis and theory aspertaining to science

How would you compare/contrast a hypothesis and theory aspertaining to science? What examples can you provide to supportyour answer?

  Principle of positive feedback

The breakdown of complex glycogen molecules into simple glucose molecules is most accurately described as a anabolic reaction.

  Calculate the average number of residues per outside loop

The maltose transporter maltoporin Mr 49,913 spans the outer membrane of E. coli. It is comprised of eighteen successive antiparallel?

  Homozygous for a mutant allele

A corn plant is homozygous for a mutant allele that results in no pigment in the seed. The mutant is caused through Ds insertion that often exits late in the seed development,

  At what position would the label reside when finished

if you have two glucose molecules each labeled with 14c at the c-2 position, feed them sequentially through glycolysis and the citric acid cycle, in what molecules and at what position would the label reside when finished?

  What is the wobble hypothesis

Why are tRNAs referred to as adaptor molecules? What aspects of their structure do tRNAs have in common.

  What is a physician trying to prevent by prescribing

AIDS is often treated with a "cocktail" of several antiviral agents at once. Why is the cocktail more effective than a single agent? What is a physician trying to prevent by prescribing several drugs at once?

  Describe the function of crop and gizzard

what type of digestive system does the earthworm have? describe the function of crop and gizzard.

  Determine the protein concentration of a protein solution

If you had to determine the protein concentration of a protein solution with a rather low concentration, which technique would you use and why?

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd