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A microcentrifuge tube with a final concentration of 0.25 mM ONPG contains 800 uL buffer, 100 uL ONPG, and 100 uL enzyme. What was the initial concentration of ONPG added to this tube?
Diagram and describe what happens to loci and alleles during sexual reproduction (meiosis & syngamy) and explain how this relates to Mendel's laws and gene linkage.
A 14-residue peptide, TRWVKVNGKWIKQA, in the context of a protein forms a hairpin, consisting of two anti-parallel ?-strands connected by a turn.
Hill buffer is a solution of 0.4 M K2HPO4 made up in 0.08M KCl. We needed 2500 mL for the week. Show your work. a.) How many grams of KCl (FW = 74.551) were needed to make this volume of buffer? b.) How many grams of K2HPO4 (FW = 174.17) were need..
List all probable points at which caffeine may be exerting its effect. Caffeine is known to alter the transmembrane movement of calcium. What is (are) the possible site(s) at which caffeine is acting.
Swelling of the abdomen and legs due to edema is characteristic of starving children with kwashiorkor, protein-deficiency disorder. What is the relationship between this symptom and procedure of capillary exchange.
All three major types of RNA are processed before they are mature. They are derived from precursor RNA molecules that are considerably longer than the final RNA product.
The intestinal stem cell sits exactly at position four in the crypt. What experiment was performed to find out it is the stem cell? At that position,
Describe the two types of RNA editing, outlining the different steps involved. Which type of editing involves the most significant changes in the mRNA sequence?
When Purified Protein Derivative (PPD) isolated from Mycobacterium tuberculosis was injected into the skin of a patient, it caused an area of hardening 1 cm in diameter. How must this PPD test be interpreted? What caused the result.
Recognizes true statements of cholesterol and its fluidity. Cholesterol increases membrane fluidity that otherwise contains mainly long chain saturated fatty acids.
Agarose gel electrophoresis was conducted on undifferentiated myogenin, differentiated myogenin, undifferentiated GAPDH, differentiated GAPDH, and a negative control ladder. The UV image of the products produced only the ladder. What could cause t..
The following information were obtained in a chemostat for the growth of E. aerogenes on aglycerol-limited growth medium.
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