Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
A few amino acids are post-translationally removed from the C-terminal end of the beta-lactamase enzyme from B. imaginarium (i.e. after translation and release from the ribosome, a protease chews off a few amino acids). The pruified wildtype enzyme is 327 amino acids in length and the sequence of its C-terminal amino acids is shown below aligned with the C-terminal aminoa cids found in a mutant strain of B. imaginarium, which contains of a frameshift mutation that sbolishes processing if its C-terminus. This frameshift mutant has had its translational reading frame altered by insertion or deletion of a single nucleotide.
wild type: S N A K D W
mutant: S N A I R L D W Q M K
a. What was the mutational event that gave rise to the frameshift mutant? Determine precisely where the single nucleotide insertion or deletion occurred in the beta-lacatamase coding sequences.
b. What is the length of the wildtype enzyme prior to processing (how many amino acids)?
c. Deduce, as far as possible, the actual sequence of the C-terminus of the wild-type enzyme prior to processing.
During translation, the mechanism of accommodation is used to select against incorrect aminoacyl-tRNAs.
Given a stock solution of 10mM, how many milliliters would you add to water to have a concentration of 5 uM (micro molar) in a final volume to 2 ml.
You are studying compound X and are trying to determine whether it is a competitive or a non-competitive inhibitor of an enzyme reaction catalyzed by the enzyme zyrase.
Critically discuss three of the test systems used to study the effects of a toxicant on chromosomes, describe each test system, and discuss one advantage or disadvantage of that particular test system.
In humans, the Xbr gene is located on chromosome 12 and is responsible for the production of a protein involved in liver function. There are 3 different alleles of the Xbr gene that exist in the human population: Xbr1, Xbr2, and Xbr3.
What are the three stages of activity in the life of an angiosperm pollen grain which succeeds in fertilizing an ovule with its sperm?
What is the metabolic exchange between rhizobia and their plant hosts? What does the plant provide in addition to carbon and how does leghemoglobin play a role in this? What metabolic feature of the bacterium is useful to the plant.
The main of eukaryotic cells are on the order of 10-100 micrometers in diameter. Bacterial cells are smaller, typically in 1-10 micrometers.
A can of Mountain Dew soda contains 46 g of sugar. Assume that your body completely digests this sugar, 170 kcal of heat energy is available from the sugar. Around how many grams of water will you have to evaporate as sweat to lose this 170 kcal of e..
Cut squares of developed film measuring 0.5cm x 0,5cm as accurately as you can. Thirty squares should be sufficient for the experiment.define Limitations of the experiment and improvements.
Which of the following statements reflects gene density.
Discussing the significance of the neurotransmitters within the neurotransmission. Describing a relationship between the dietary fat intake and related health concerns.
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd