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In mapping a large plasmid you notice that the restriction enzyme HaeII yields three bands, Pst I yields five bands and HaeII+PstI double digestion gives rise to six bands
A) What is the inconsistency of these data and what are two possible explanations?B) How could you distinguish between these possibilities from scrutiny of the gel electrophoresis results?C) You want to ligate the largest fragment generated by PstI with the largest fragment generated by HaeII. However the ends that are generated by the two enzymes are not complementary. What would you need to do in order to carry out the ligation?
What is the pI of leucine, Draw the condensed structure for leucine, and label all chirality centers with an asterisk
Think about a man who has been exercising. His arterial receptors detect a rise in his blood CO2 and send this information to the brain. He requires to increase both the rate and depth of his breathing.
explain how stem cells obtained from fertilization in the fallopian tube and somatic cell nuclear transfer differ in terms of the source of their genome, age of blastocyst.
In a cross in Drosophila, a female heterozygous for the autosomally linked genes a, b, c, d, and e was testcrossed with a male homozygous for all recessive alleles.
Explain each of the stages of viral reproduction: Absorption Penetration Synthesis Maturation Release
What happens if intrapleural pressure becomes equal to atmospheric pressure. McCloskey implies that evolution has displaced the need for a designer.
Draw a phospholipid containing palmitic and linoleic acid with the unsaturated fatty acid at C-2 of glycerol. To the phosphate, esterify a molecule of choline. Indicate which portion of the molecule would be in the lipid bilayer and which would be..
Explain how the two different kinds of mountains are formed by plate movement. Be sure to mention how the density of the plates affects what kind of mountains form.
How should the area of the forest patches in an agricultural landscape affect the proportion of bird species in a community that are associated with forest edge habitats?
Insulin is released from pancreatic b cells when blood glucose levels increase and glucagon is released from pancreatic a cells when blood glucose levels decrease.
Receptor ligand interactions, the researcher wanted to narrow down exact region of binding between the receptor and the ligand.
More than half of the world's species of plants and animals are found in the rainforests. For this reason, we tend to think of them as very fertile areas and they have been called the "jewels of the Earth". Despite the rapid vegetation growth and ..
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