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A protein has a molecular mass of 200 kDa as determined by gel filtration (=native molecular weight). When separated by SDS-PAGE (without a reducing agent present) only one polypeptide appeared with a mass corresponding to 100 kDa. When determined by SDS-PAGE in combination with a reducing agent (DTT) exactly two polypeptides appeared of sizes 40 and 60 kDa. What is the correct structure of the native protein?
Select one:
a. The native protein is made of one 40 kDA and one 60 kDA polypeptidesb. The native protein is made of two 100 kDa polypeptidesc. The native protein is made of two 40 kDA and two 60 kDA polypeptidesd. The native protein is made of two 100 kDa polypeptides, one 40 kDA and one 60 kDA polypeptidese. None of the answers is correct.
In a population with two alleles, A and a, frequency of A is 0.2. Organisms that is homozygous for A die before reaching sexual maturity. In five generations, what will be the frequency of individuals aa with genotypes.
Determine which population size exhibited the greatest amount of variation in allele frequency during these stimulations?
Currently, three fossil skulls of Homo sapiens, dating to about 160,000 years BP, were discovered in eastern Africa. These skulls fill a gap between the 100,000 year old H. sapiens.
E.coli and S.epidermidis were chosen to represent Gram-negative and Gram-positive bacteria, respectively. For a given antibiotic, is there a difference in susceptibility between the Gram-positive and Gram-negative bacteria?
which of the following would you expect to find in normal urine?
What is Red Queen evolution and how does it apply to the search images of predators and the camouflage patterns of prey? Discuss in the context of specific experimental studies.
The muscles that originate on the ischium and sacrum are" Adductors of the thigh. Abductors of the thigh. Medial rotators of the thigh.
In a study of 600 people, 345 have Rh+ and 255 were Rh-. Assuming Rh+ is dominant allele D and Rh- is recessive d, calculate the expected number of Rh+ people who are heterozygotes.
Calculate the total number of ATP molecules that will be produced upon complete breakdown of C14 fatty acid molecule. Show complete details of your calculation.
A double-stranded fragment of viral DNA, one of whose strands is shown below, encodes two polypeptides called vir-1 and vir-2. Adding this double stranded DNA fragment to an in vitro transcription and translation system yields
What is the explanation for the fact the most cells are small and have cell membranes with many convolutions.
In the past, people infected with Hansen's disease were committed to leprosariums to prevent the spread of the disease. Explain why do you think that once treatments became effective.
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