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Viral vectors are one method commonly used for gene therapy. Two types of viral vectors are retroviruses or adenoviruses.Regarding the delivery and expression of the engineered gene, how do these two systems differ?
Assuming you are able to eliminate the negative reactions associated with viral therapy, pick a disease state you would treat with a retrovirus and one you would treat with a adenovirus, and describe why you would pick those particular diseases.
You would like to genetically engineer a bacteria so it will glow in the presence of an environmental contaminant. The bacteria you select has the ability to grow in the presence of the contamination and secretes a specific enzyme in response to the contamination. You decide to use a fusion protein based system.
What is a fusion protein and how would you construct it.
Once you have your fusion protein you will need to insert into a plasmid which will serve as the reporter mechanism. Describe how you would construct this plasmid (describe the promotors, binding sites not the actual molecular techniques used to construct the plasmid) in order make the bacteria glow in the presence of contamination.
Why does the loop of henle have the long narrow shape? Some desert-adapted animals produce a very concentrated urine. How are their kidney different?
The rule of thumb concerning ecosystems as units that capture energy and transport it is as follows: For every jump in trophic level, the amount of available energy drops by 90%. Discuss in detail what accounts for this loss of energy? Please give..
Determine the forces that cause the change in filtration rate as plasma passes through the capillary?
While reading, pay close attention to the various ways in which an agreement may not be legal, including instances of usury, gambling, violation of Sabbath laws, agreements made to commit a crime or tort, and agreements made by a party without proper..
Which of the following statements is *NOT* true?
What is the probability that an individual haploid resulting from the meiotic division will have a complete parental set of centromeres (that is, a set all from one parent or all from the other parent)?
A profressor in a second-term calculus course wants to determine whether the year in college has any effect on the performance of his students on their final exam.
The chemical equation FeS --> Fe + S is anexample of this kind of reaction. Potential energy can be illustrated by which ofthe following examples?
Which disinfectant was the most effective against theorganism and explain. Can you determine whether compound Y was bactericidal orbacteriostatic?
You are trying to find a restaurant to take your great grandmother for Mother's Day. You have taken her to several places in town, but she is rarely satisfied with the food she is served, complaining that the food lacks flavor.
q1. what kind of cell communication is said to ovvur if the cell sevretes the growth factor that then acts on
How would you prepare a 250 ml solution, 0.5 M in sodium phosphate buffer at pH of 7.0. You have in the laboratory monobasic sodium phosphate (NaH2PO4) and diabasic sodium phosphate (Na2HPO4). pKa = 7.2.
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