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Consider a culture into which two E. coli strains are inoculated. Strain A is normal with g=21 min at 37 degrees C, and 42 mins at 27 degrees C. Strain B has a temperature-sensitive mutation, such that g=55 mins at 37 degrees C, and 39 mins at 27 degrees C.
We inoculate equal amounts of cells (1 ml of each starter culture whose population is 1 x 10^6 cfu/ml) into each of two separate 1 liter volumes of LB broth at time=0. One of the flasks is incubated at 37 degrees C, one at 27 degrees C.
Assuming constant exponential growth (i.e. no lag phase, and no exhaustion of nutrients), answer each of these questions:
1) After 6 hours of growth, what is the final concentraion of each strain in each flask?
2) What fraction of the total population is Strain A and Strain B in each flask?
3) How long would each flask need to go for one strain to become 90% of the population?
Write down an equation for the complete oxidation of glucose. What does this imply about the volume changes you would view if you simply sealed the pea seeds in a flask.
Suppose that the cells grow to about 10^5 cells per squre cm and contain about 10pg RNA per cell, determine the theoretical yield of RNA from one of your samples.
What fraction of offspring of the cross AabbDd x AaBbdd are expected to have the same phenotype as either one of their parents assume that all three genes are on different chromosomes.
Identify the 6-main skeletal muscles and tell how they function. There are so many of them, I couldn't find out which are the six majors.
Examine the feedback mechanisms that maintain homeostasis what happens when these systems do not function appropriately.
Compare the approaches taken by Kepler and Newton in developing their theories. What is the difference between discover and derive? Note that in the process of developing their theories both of them broke new ground in methods of scientific inquir..
During a cross between organisms having genotypes of AaBbCc and AaBbcc, what proportion of the offspring would be expected to be aaB_Cc?
Explain 4 mechanisms by which the integument helps to prevent infection. In other words, explain either specific actions the integument performs or characteristics of its structure.
Cystic fibrosis is a chronic lung and digestive disease caused through a mutation in the CFTR gene on chromosome 7. In the US approximately 39 in 10,000 individuals are affected.
Pyrethrum is an African plant that develops daisy-like flowers with white petals and yellow centers. The chemical pyrethrin is produced in large amounts in the flower buds.
We now understand that mutations that cause the inhibition of apoptosis are found in tumors. Because proliferation itself is not induced by the inhibition of apoptosis, explain how this inhibition might contribute to tumor formation.
Present two factors contributing to the solid waste problem in the U.S. How could these factors be changed or controlled for the better? What problems are there with current recycling efforts? What is being done in your area relative to recycling?
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