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You are doing a PCR that requires 1.6ng of DNA in 2uL of sample. You have 100uL of stock DNA with a concentration of 4ng/ul. Using reasonable pipetting volumes (ex. don't use too much of your sample but don't pipette an unreasonably small volume), how would you dilute your stock DNA sample to meet this criteria? Use the procedure in Exercise #3; show all formulae, variables, units and calculations.
What is a multiplex PCR? What differs in this reaction from a traditional PCR?
How are PCR products fluorescently labelled in the Identifiler Kit? Name four dyes used in this kit and their corresponding colours.
What standard is used during electropherogram interpretation to determine the size (in bp) of a peak given its electrophoretic mobility?
What reference standard is used during electropherogram interpretation to score alleles given the size of a DNA fragment?
Define incomplete 3' addition. How does this appear on an electropherogram?
What causes stutter? In the Identifiler kit, what is the most common size difference (in bp) between the true allele and the resulting stutter product? What does this difference represent?
Suppose a membrane potential of -70mV, what are two reasons why the electrochemical gradient for Ca2+ ([10-7 mM] free in the cytoplasm
Although animals do not have glyoxylate cycle its is still possible to find out radioactively labelled carbon atoms from triacylglycerols incorporated into glucose.
If you were modeling salamander population growth with the logistic growth equation, during the first few years: Pick one of the options below a.) N
What is the total number of noncovalent bonds joining the nucleotides of the complementary strands?
A brief introduction about "loblolly pine","Pinus taeda". What does these terms mean in Ecology?
Assume you are studying genes and DNAsequences involved in the regulation of the grandoperon. When your lab's strain of e. coli are in the presence of the sugar "grandiose," transcription of 2-enzymes grandAand grandB increases.
Calculate the fraction of the total enzyme active sites in solution that would be in the active form at pH 7.2. Calculate what kcat would be if the activity of the enzyme were measured at pH 7.2.
All of the following functions are incorrect for the nasal conchae.
1000 molecules are bouncing at 300K in two wells separated by a free energy gap DG = 6·10-21 J. Compute how many particles on average are in each well.
discuss the different phases of the cell cycle. at which part of the cell cycle would you expect that a change in
q1. monarch butterfly populations are found east and west of rocky mountains. how can dna fingerprinting technology be
Is ecdysone secretion from the prothoracic gland of insectscontrolled by feedback inhibition of the neural structures that secrete brain hormone?
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