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A standard procedure in purifying tubulin is to extract the tissue on ice, then add GTP and warm the tissue extract to 37°C. The warmed extract is then centrifuged at a speed insufficient to pellet average sized proteins, but sufficient to pellet large protein complexes. After centrifugation, the supernatant is discarded, and the pellet is redissolved in cold buffer. GTP is again added to the redissolved pellet proteins, and the solution is again warmed for a few minutes. Then centrifugation at the same speed described above is again performed. This process is repeated several times.
1. How would this process result in purification of tubulin?
2. What contaminating proteins would likely co-purify with the tubulin?
A fish on the outside of a school swims inward, leaving other fish on the edge. These new "edge" fish then move inward and the cycle repeats.
Describe how a CAM plant responds to hot, dry weather. How does differ from the C4 plant ?
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The symptom of numbness of the palmar region often associated with carpal tunnel syndrome is caused by: Compression of the long extensor muscles of the hand. Compartmentalization of flexor and extensor muscles.
A three-factor cross in Salmonella typhimurium was performed using bacteriophage P22. The cross was between an arg-leu-his- recipient bacterium and bacteriophage P22.
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A 25-year-old graduate student presents to the university health center complaining of acute shortness of breath. The patient states that he returned home yesterday from a non-stop flight from Sydney, Australia.
Assume that this population is undergoing mutational pressure. The rate of mutation is 1.0 x 10-4 for changing A to a and 1.0 x 10-5 for changing a to A. What is the allele frequency of A after a single generation? (do not round your answer)
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Trace path from the femoral vein in a healthy female to the lower lobe of the right lung via the right pulmonary artery. Explain all major structures on the way.
Determine the time domain equation that describes the change in drug concentration, C(t) for a given step increase in infused drug concentration, M?
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