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1. Biologists frequently use phosphate-buffered solutions in experiments. You need to prepare a sodium phosphate solution at pH 6.7 by mixing NaH2PO4 and Na2HPO4 (pKa =7.2). What are the respective concentration of each of these substances if you want the final concentration of phosphate ([HPO4-2]+[H2PO4-] to be 150 mM?
2. Activity of the bacterial enzyme acetoacetate decarboxylase sharply declines below pH 6, suggesting there is an ionizable catalytic residue with pKa ~ 6. Using mutagenesis, this critical catalytic residue was determined to be Lys-115.
a) For catalytic Lys-115, what is the ratio of deprotonated to protonated side chain at pH 7.5?b) The neighboring residue at position 116 is also lysine. Explain how this perturbs the pKa of the catalytic lysine residue.
A standard basketball has a volume of 7.47 L. What is the volume of the basketball in gallons? The speed limit on many United States highways is 65.0 miles per hour
solid sodium sulfate reacts with solid carbon to form solid sodium sulfide and carbon monoxide. if 15 grams of sodium
A sample of argon has a volume of 205 cm3 when its temperature is -44.0 oC and its pressure is 712 mm of Hg. What would be the volume of the argon at STP?
Briefly describe the adverse effects caused by exposure to radiation. Include some specific exposure levels associated with these significant health effects.
ethanol vapor at 0.5 bar and 78 degrees celcius is compressed isothermally to 2 bar in a reversible process in a closed system a) draw a qualitative PV graph and show the path of the process.
An oxygen tank contains oxygen at a pressure of 70 kPa. When the pressure drops to 36 kPa, the temperature increases from 300 K to 350 K. If the initial volume is 9 L, what is the final volume?
A particular enzyme catalyzed reaction was followed and data collected as indicated below. Using the data, determine the Michaelis constant and the maximum velocity of the reaction.
calculate how many mL of 0.200 M NaOH (aq) would be needed to react with the malic acid sample from step c.
The nitrate ion (NO3-) is a very poor nucleophile. In fact, solvents such as water or methanol (also weak nucleophiles) are more reactive nucleophiles than the nitrate ion
Explain in terms of their electron configurations, why Fe2+ is more easily oxidized to Fe3+ than Mn2+ is to Mn3+.
When a voltage of 30 V is applied to the heater for 3.0 min, a constant current of 2.5 A is observed. The calorimeter temperature increases from 20.00C to 25.59C. Calculate C_bomb for the calorimeter. Assume 2000.0 g of water in the bucket.
Calculate the vapor pressure of a solution containing 29.4 g of glycerin (C3H8O3) in 120 mL of water at 30.0 C. The vapor pressure of pure water at this temperature is 31.8 torr
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