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1. Biologists frequently use phosphate-buffered solutions in experiments. You need to prepare a sodium phosphate solution at pH 6.7 by mixing NaH2PO4 and Na2HPO4 (pKa =7.2). What are the respective concentration of each of these substances if you want the final concentration of phosphate ([HPO4-2]+[H2PO4-] to be 150 mM?
2. Activity of the bacterial enzyme acetoacetate decarboxylase sharply declines below pH 6, suggesting there is an ionizable catalytic residue with pKa ~ 6. Using mutagenesis, this critical catalytic residue was determined to be Lys-115.
MCB354FA15_PS01
a) For catalytic Lys-115, what is the ratio of deprotonated to protonated side chain at pH 7.5?
b) The neighboring residue at position 116 is also lysine. Explain how this perturbs the pKa of the catalytic lysine residue.
Assume that there are 12 map units between two loci in the mouse and that you are able to microscopically observe meiotic chromosomes in this organism. If you examined 200 primary oocytes, in how many would you expect to see a chiasma between the ..
Under certain conditions, the rate of mutation of a particular gene may be determines in humans. What properties of the mutation would favour the most direct determination of mutation rate in humans? Write three points.
Also, explain the difference between pasteurized processed American cheese, pasteurized processed American cheese food, and pasteurized processed American cheese product?
Which of these is the largest terrestrial biome on Earth? A) tundra B) coniferous forest C) temperate broadleaf forest D) temperate grassland E) desert.
Describe how vestigial structures provide clues about a species' evolutionary past. Illustrate the argument with vestigial structures found in humans or other real species.
Suppose if equal amounts of Na2HPO4 and NaH2PO4 are mixed in water, compute the resulting pH. The pKas of phosphoric acid are 2.1, 7.2, 12.4.
What is rabies? What animals carry it? What are ways to prevent it? What about the vaccine? Who should get it? answer should be about 200-300 words and should be written clearly.
determine the number of significant figer in the following measurment [ 12.3 ml and 2.3 g and 5.877 micrometer] then round the number 56.877 to the following number of significant figures [4 and 3 and 2]?
Design an experiment to determine order of action of genes ETR1, CTR1 and EIN in ethylene signalling? Also design an experiment to see whether ETR1 interacts with CTR1 or not?? give a brief overview of the experiments.
An operon with one regulatory region and three protein?coding genes will have how many promoters? Related: Explain how bacteria can make one mRNA molecule that contains the code for several proteins.
Assume that you have a stock solution of epinephrine at a concentration of 1mg/ml. Further suppose that there are 20 drops/ml.
After fiddling with the simulation program to see how it works, restore all parameters to their default settings.
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