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In a eukaryote, transcription takes place in the cytoplasm (prokaryote) or in the nucleus (eukaryote). The transcription is performed by an enzyme called RNA polymerase. To make mRNA, RNA polymerase:
mRNA is happy to live in a single-stranded state (as opposed to DNA's desire to form complementary double-stranded helixes). In prokaryotes, all of the nucleotides in the mRNA are part of codons for the new protein. However, in eukaryotes only, there are extra sequences in the DNA and mRNA that don't code for proteins called introns.
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