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You believe that the cytosolic protein p007 is involved in mediating growth hormone-stimulated proliferation in cells. You wish to establish a cell model using your cultured fibroblasts. Provide clear explanations to the following.
a) Describe how you would transfect the growth hormone receptor DNA into your cells to create stable cell lines, and include a description of the benefits of using stable cell lines versus transiently transfected cell line.
b) Describe how you would measure specific receptor binding of GH to your stable cell line.
c) Describe how you would measure growth hormone-induced proliferation in your stable cell line. Make sure to include controls.
d) Describe how you would use RNAi to demonstrate the requirement for p007 in growth-hormone stimulated proliferation. Make sure to include controls.
Use the differences in how sperm and ova develop during meiosis and explain why human mitochondrial DNA represents only the maternal, and not the paternal, human genetic lineage.
Valerie is a 15 year old girl who has recently had signs of a high fever, What disease does valerie have
Another important component in this course is the Reaction Paper. The drop box for the paper opened at midnight on the first day of Week 2 so you can begin working on your paper. There are detailed instructions for the paper in the Paper folder (C..
q1. in adding to the law of conservation of matter we require to understand the first and second laws of
Plant: gasteria, agave, Give a brief overview (several paragraphs each) on the folklore and medicinal uses of the plants you find. Required: 1000 words, works cited.
Myelin is important for action potential propagation. Multiple sclerosis is an autoimmune disease where the myelin sheath surrounding CNS axons is progressively lost. How will this degradation affect the generation of action potentials?
There are four types of inheritance patterns in pedigrees. Name them. What would you expect a pedigree to look like for each pattern
Where are the male and female gametophytes located on a Peyote(Lophophora Williamsi)?
contrast somatic cells and gametes with respect to their ploidy (diploid v. haploid), number of chromosomes in humans, and locations in the body of organisms
Meiosis explains Mendel's laws. Explain the phenotype of a mutant in the cell cycle that would disrupt Mendel's first law
For each of the bags, identify whether the solution inside was hypertonic, hypotonic or isotonic in comparison to the beaker solution it was placed in.
Which of the following is/are part of the endomembrane system?
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