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To make a phosphate buffer at pH 12.38 starting with one liter of 10mM phosphoric acid (H3PO4; pKs are of 2.15, 6.82, and 12.38), you could add:
a) 15 millimoles of KOH
b) 20 millimoles of HCL
c) You cannot make a buffer by adding HCL or KOH
d) 25 millimoles of HCL
e) 25 millimoles of KOH
Before freezing, you count your cell suspension in the presence of trypan blue. The initial volume of thecell suspension is 10ml. You add 0.1ml of the cell suspension to 0.1ml of trpan blue.
Discuss the the importance of translational control in early development in animals. Discuss how the experiments on matroclinous inheritance (maternal-effect genes) elucidate the linkage between cleavage events and later development.
What is the M locus, D locus, and Bk locus, in terms of the relationship among genes, alleles and chromosomes.
Perform a search to find out amino acid sequences of the database similar to the query amino acid sequence supplied. explain a BLAST Search Query Amino Acid Sequence.
Which cytoskeletal proteins are important constituents of the contractile structures that form the cleavage furrows involved in animal cell cytokinesis?
In addition, the L,l allelic pair is subject to abnormal transmission ratios in males only, such that heterozygous males transmit 70% l-bearing sperm and 30% L-bearing sperm.
Define gene expression and explain how the information contained in DNA lead to thecreation of living things. List the conclusions Griffin, Avery, Hershey, and Chase drew from their experiments.
Human Papilloma Virus. Read this review on how HPV controls the expression of its genes and explain to the class how HPV uses splicing and polyadenylation to regulate production of viral proteins.
List and describe at least three cellular features of bacteria that could be targeted to inhibit or kill bacterial pathogen. Which is a significant flaw in this experimental set-up.
Palpitation of both carotid arteries at the same time initiates a reflex known as the baroreceptor reflex. If this reflex is initiated in a healthy person with normal blood pressure, the person may temporarily lose consciousness. Explain why this ..
Polio and HIV are both +ssRNA viruses have very different strategies for replicating their genomes. After the viral genomes enter the cell, explain the first step involved in replication of each genome.
What series could you use to make a beta barrel with twelve antiparallel strands? Write out one repeat of the sequence as follows: M-(your sequence) 12.
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