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Suppose that an enzyme uses a catalytic mechanism in which a functional group on the enzyme has to accept a proton from the intermediate formed in the first chemical step. The pKa of that basic functional group on the enzyme is 8.0, and is the only pKa apparent on a plot of kcat vs pH for the activity of this enzyme. The kcat of the fully active form of the enzyme is 1 X 10 ^5 sec^-1..1. What will a graph of the effect of pH on the activity of this enzyme look like? (Graph of kcat vs pH)2. What type of functional group might be responsible for the pH effect on kcat? Justify your anser based on the generic pKa values of functional groups in peptides and proteins..
q. in a mutant strain of e. coli a trna that recognizes the codon 5 cug 3 of normal cells has a point mutation so that
Which of the following tasks CANNOT be performed by earthworms?
A violation of which assumptions will result in a change in the allele frequency and determine which population most likely has assortative mating?
There are two general mechanisms of miRNA/AGO complexes. If miRNAs are fully complementary with mRNAs, AGO can cleave these targets. If there are mismatches between the middle positions of miRNAs and their targets, then the miRNA/AGO complexes can..
Describe three differences between RNA and DNA with respect to their chemical composition and structure. Provide a detailed description of each characteristics you chose in your response.
Observe the life table of an arthropod. Is this population increasing or decreasing? If the population becomes endangered, at what point should the organism be carefully protected?
Considering the circumstances and the preliminary work-up, what type of anemia is most likely for Ann? Which clinical sign shows her body is attempting to compensate for anemia?
an enzyme lab you will do later this term measures production of yellow colored nitrophenol over time. what range of
q1. your roommate says that the concept of homeostasis is being violated while the rate of respiration goes up during
What do you predict will happen to pulse amplitude when you raise your hand and what can you conclude about the effect of altering hand position on pulse amplitude
You have stock solutions of 10 M KCl and 100 mM HEPES buffer, pH 7.5. You are asked to prepare a 100 mls of 100mM HEPES buffer containing 0.35 mM KCl. How would you make this buffer solution?
Several main types of animals are now extinct. A treasure trove of fossils from these unusual animals was found in the Canadian Rockies.
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