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You believe that the cytosolic protein p007 is involved in mediating growth hormone-stimulated proliferation in cells. You wish to establish a cell model using your cultured fibroblasts. Provide clear explanations to the following.
a) Describe how you would transfect the growth hormone receptor DNA into your cells to create stable cell lines, and include a description of the benefits of using stable cell lines versus transiently transfected cell line.
b) Describe how you would measure specific receptor binding of GH to your stable cell line.
c) Describe how you would measure growth hormone-induced proliferation in your stable cell line. Make sure to include controls.
d) Describe how you would use RNAi to demonstrate the requirement for p007 in growth-hormone stimulated proliferation. Make sure to include controls.
Assume you are studying metabolic pathways and discover that 2-pathways intersect so that enzyme basinase participates in both of the intersecting pathways.
Consider the list of scientists that have been awarded a Nobel Price for their work in immunology which one would you consider being the most important work and why?
q1. dna fingerprinting identifies individual bears and also allows for determining gender of the bear. what information
Many organisms possessing many different lipases produce positive results on Tributyrin Agar. Is the inability of this medium to distinguish between these different enzymes a weakness in its specificity or its sensitivity?
TSIA and KIA are complex media with many ingredients. What would be the consequences of the following mistakes in preparing this medium? Consider each independently.
q. assume that pesticide resistance in beetles is a quantitative trait that can be measured as the number of days a
Ear length in long-eared bunnies is inherited as a quantitative trait. When two heterozygous bunnies are crossed, a normal distribution of 5 different phenotypes is observed in the progeny in a ratio of 1:4:6:4:1. How many contributing alleles are..
What is the cellular tropism of HIV and does it change during the course of an infection? Which cellular receptors confer the preference for infection of certain cell-types?
Which of the following is true of CD4+ lymphocytes.
More than half of the world's species of plants and animals are found in the rainforests. For this reason, we tend to think of them as very fertile areas and they have been called the "jewels of the Earth". Despite the rapid vegetation growth and ..
q1. imagine that you perform fruit fly crosses with dissimilar pairs of three x-linked traits specified by genes x y
Genes a and b are 20 cM apart. An a+b+/a+b+ individual was mated with ab/ab individual.
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