Southern blot to detect the junction fragment

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Reference no: EM1386559

Diagnosis of CML can be done using a Southern blot to detect the junction fragment. In the early stages of CML most patients are heterozygous for the genome rearrangement, while at later stages homozygosity is observed. Using a probe that detects both the junction fragment and the normal cABL gene, would you be able to distinguish between someone that is heterozygous and someone that is homozygous?



Reference no: EM1386559

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