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A plasmid 3 Kb in length contains a gene for ampicillin resistance and a gene for tetracycline resistance. The plasmid has a single site for each of EcoRI, Bg/II, and PstI. If DNA is cloned into the EcoRI site, resistance to either antibiotic is not affected. DNA cloned into the PstI site eliminates ampicillin resistance.DNA cloned into the Bg/II site abolishes terracycline resistance. Complete double digestion fragment produced are as follows: EcoRI + PstI=2.3 and 0.7 Kb; EcoRI + Bg/II=2.77 and 0.3 kb. Draw a restriction map of the plasmid, showing the locations of the antibiotic resistance genes and the restriction enzyeme sites.
Explain why are ripe fruits often colorful, fragrant and sweet? Determine the difference between pollination and fertilization in flowering plants?
A breaking buffer contains 10mM Tris, pH 8.0, 150mM Nacl. The elution buffer is breaking buffer that also contains 300mM imidazole.
At the starting of the spring, Dr. Betty Burner notices that there is an equal distribution of long and short-stemmed dandelions in her backyard.
Plants lacking this enzyme grow slowly and are often light green or white in colour. Provide explanations with support for both these plants' slow growth rates and light-green or white appearance.
Eight year old flown into emergency center from rural hospital. He was helpping his father out on a farm. The sketchy history obtained was that the child developed nausea and vomiting.
Explain why there are no male offspring with the allele combinations
Explain a specific example of a cross between two parents, each homozygous for different traits, which would result in a phenotype that is distinct from either parental phenotype.
At the startingof the spring, Dr. Peter Erlenmeyer notices that there is an equal distribution of long and short stemmed dandelions in the vacant lot across the street from his house as well as in his own backward and Explain how the information stor..
In order for the 4-nitrophenol to release the yellow colour expected, it has to be fully ionised. With a pKa of 7, I have decided that pH9 will allow it to be fully ionised - this is also supported by yellow colour given off, and a reasonable calib..
Monarch butterfly populations are found east and west of Rocky Mountains. How can DNA fingerprinting technology be used to establish that they constitute one breeding population or two separate breeding populations.
What is "Independent Assortment" of chromosomes? Who is associated with this theory? Throughout what phase of Meiosis I does it happen.
A clinical microbiologist makes serial dilutions of several antimicrobials in broth, and then incubates each drug dilution series with a standard amount of a patient's isolated pathogen. What is this micro biologist setting up.
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