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1. This method can be used to serilize a liquid media without harmingheat labile ingredints:a. autoclavingb. pasteurizationc. freezingd. cold filtratione. addition of ethylene oxide to the media2. Consider two parallel cultures. They are in all ways identicalexcept that one contains test substance X. You observe that abacterium you are studying grows equally well in both media.Therefore, test substance X is ___________.A macronutrientA micronutrientAn essential nutrientA toxinNot a nutrient3. A microbe uses CO2 as its sole source of carbon and oxidizes H2 asits source of energy, to which nutritional group does this microbebelong?PhotoautotrophChemoorganotrophPhotoorganotrophChemoautotroph4. A mutant bacterium does not produce a functional hydroxamate. Thisbacterium would have difficulty obtaining ___________.PhosphateIronAmmoniaThiosulfateHydrogen sulfide5. A medium containing methylene blue and incubated at 55º Cenriches for ______.Mesophilic mycoplasmaHyperthermophilic gram negativesGram positivesThermophilic gram negativesThermophilic gram positives6. Bacterium “A” has a growth rate constant of 4doubles/hour. Bacterium “B” has a generation time of0.25 hours/double. Which bacterium grows faster?“A” grows faster than “B”“B” grows faster than “A”A” and “B” grow at the same rateNo conclusion can be made from this data7. Assume you inoculated 100 cells into 100 ml of nutrient broth. Youthen inoculated 100 cells of the same species into 200 ml ofnutrient broth. After incubation for 12 hours, you should haveMore cells in the 100 mlMore cells in the 200 mlThe same number of cells in both.8. _________ halodurics use K+ as their osmolyte.AllSomeNo9. A medium containing 3.2% NaCl and 0.025% phenethyl alcohol wouldenrich for _________.NonhalophilesGram-negative haloduricsGram-negative halophilesGram-positive hyperhalophilesGram-positive halophiles10. The value of the minimum inhibitory concentration dependsupon_________.The microbe’s intrinsic sensitivity to the antimicrobialThe rate at which the antimicrobial diffuses through the agarBoth A and BNeither A nor B11. Which enzyme would be used to neutralize the activity of the[OH·] free radical?Superoxide dismutaseCatalasePeroxidaseNitrogenaseNone of the above12. Which of the following can sterilize?70% ethanol10% sodium hypochorite solutionEthylene oxideMecurochromeAll of the above13. You are developing a new line of protease containing laundrydetergent. You plan on marketing the detergent to the NFL to removegrass and blood stains from their uniforms. You most likelyisolated the protease from which group of bacteria?AcidophileNeutrophileAlkalophileStrict anaerobesNon-halophile14. True or False: Fungi are better osmophiles than most bacteria.TrueFalse15. The cell density of population of microbes expands six-thousandfold. Approximately how many times did the population double?91012141616. Thirty-six colonies grew on nutrient agar plate from 500μl ofundiluted sample in a standard plate count. How many cells / mlwere in the original sample?17,000900183672
Are Pat and Robin's parents both normal or does one of them have Angelman or Prader-Willi syndrome? If one of them has a disorder, explain why it is the mother or the father.
Assume y ou are analyzing a dominant allele in fruit flies that mutates to a recessive allele at the relatively high rate of 10 -6 per generation.
show how an increase in the quantity of medical care consumed can be achieved through either a demand or supply
How is it possible that mrna synthesis occurs at a greater rate in bacterial cells than any other class, yet very little mrna is present within the cell.
The only instruments available to you are only a PCR instrument, gel electrophoresis equipment, and DNA sequencing instrument.
perform a literature search on human resource law or policy.prepare a 6-10 slide powerpoint presentation with speaker
Po2 (torr) has the following values 1,5,10,15,20,25,30,35,40,50 Y has the following values 0.001, 0,09, 0.36, 0.60, 0.78, 0.86, 0.91, 0.93, 0.95, 0.97. Graph the data above as a hill plot and estimate the "n" value of mutant hemoglobin.
NADH Passes it's electrons on to oxygen through a long chain of electron carriers. How much free energy (in kj/mol) can in principle be obtained form this process under standard conditions (pH7) for each mole of oxygen that is reduced to water?
Which type of transport functions to make a concentration gradient ?
Calculate the net ATP yield (ATP equivalents) for the combined complete oxidation of 1 molecule of serine and 1 molecule of alanine to CO2 in the liver, taking into consideration the energy cost of nitrogen excretion as urea.
Discuss similarities and differences of the nitrogen requirements of microorganisms and higher forms of life, describing how their respective requirements are met.
Application of an insecticide to control mosquito populations in malaria infested areas quickly resulted in a dominant mutation, R, resulting in insecticide resistance.
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