Samples of nucleic acid and chemical identity

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1. A friend brings you three samples of nucleic acid and asks you to determine each sample's chemical identity (whether DNA or RNA) and whether the molecules are double-stranded or single-stranded. You use powerful nucleotides and then determine the approximate relative proportions of nucleotides. The results of your assay follow. What can you tell your friend about the nature of these samples?
Sample 1: dGMP 13% dCMP 14% dAMP 36% dTMP 37%
Sample 2: dGMP 12% dCMP 36% dAMP 47% dTMP 5%
Sample 3: GMP 22% CMP 47% AMP 17% UMP 14%

2. A suspension of E. Coli was serially diluted through two dilutions of 100-fold each and two dilutions of 10-fold each. From the final dilution, a volumn of 0.1 ml was spread over nutrient agar in a petri dish and incubated overnight. The next day, 42 colonies were visible. Estimate the number of viable bacteria per milliliter in the original undiluted suspension.

Reference no: EM13196540

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