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1) Represent graphically the relation between melting temperature and the G-C content of DNA.
a) Construct relations for dependence of melting temperature of a 30 bp DNA duplex on the G-C content. For series of the DNA chains where we vary the number of G-C pairs, plot how Tm changes as a function of f, the fraction of G-C pairs, ranging from 0.33 (i.e. 10 G-C pairs) to 0.67 (i.e 30 G-C) pairs.
b) From observation, list other factors other than G-C content seem to control Tm?
c) Supposing we have two actin filament 10 microns long linked by 10 myosin motors, how long would be needed to move two filaments past each other 5 microns? How much energy is needed?
Assume that decomposers were removed from the forest ecosystem; predict what would happen to the rate of nitrogen cycling. Explain the logic behind your prediction.
What chemical property of the leucine residues thought to form tight ring that prevents the passage of ions within the channel of the acetylcoline receptor suits them for this role.
If the experimental animals in a chamber were engaged in physical activity, how would this change the results of metabolism experiment.
The best characterization of apoptosis is: the unwanted, unexpected death of a cell. cell death is a normal consequence of a cell living long enough to eventually be killed by accident.
Why the parents wake up, other than the children did didn't wake up. A gene for sweat gland production is found on the X chromosome.
A nearsighted woman (Nn) with hazel eyes (Hh) marries the man with normal vision and hazel eyes (Hh). Their three children all have blue eyes and normal vision. What is the possibility that their next child will have blue eyes and be nearsight.
What do you think is the most probable mechanism of browning? If a patient's resting cardiac output is 5.6 l/min and on a stress test she elevated her heart rate to the highest of 176 beats/min with a stroke volume of 115 ml/beat, what is her cardiac..
What may have been the cause of this disease that generally strikes a person much older than Hank. Flask A contains yeast cells in glucose-minimal salts broth incubated at 30 C with aeration. Flask B contains yeast with cells in glucose-minimal salts..
Another dog, Billy, has three restriction sites for this enzyme in his genome. For this problem suppose there is only one chromosome in dogs.
Illustrate the process of diffusion. Name and explain the condition that exists when the diffusion of a particular substance is complete.
Muscle cells, while an individual is exercising heavily and at what time the muscle becomes oxygen deprived, convert pyruvate to lactate. What happens to the lactate in skeletal muscle cells.
Your friend is trying to generate an in vitro system for studying the formation of COPII vesicles. He finds that to get well-organized vesicle budding from artificial membranes, he needs to add the coat proteins, the Sar1 GTP-binding protein.
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