Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Task: Coould you re-write this abstract in a scientific manner, as in how an abstract would be written in a research paper.
Title: Could Sonic Hedgehog signaling increase Bmp expression?
Abstract
Following a previous study carried out by a number of PhD students from university of east London it was demonstrated that the activation, proliferation and survival of stimulated B cells increases at 18 hrs and decreases at 40 hrs. this study therefore was done to investigate the mechanism in which is responsible for the negative effect at 40 hrs. it was hypothesized that Shh protein could increase BMP-2 and BMP-4 expression at 40 hrs as these bone morphogenic protein have previously been identified as antagonist of B cell activation.Purified B cells from the spleen of female BALB mice were stimulated by anti-IgM and anti-CD40 and mixed with 0.5 and 0.05 μl/l of recombinant Sonic hedgehog protein in duplicates in two separate plates. The plates were then incubated for 18 and 40hrs. after each time set cells populations were analyzed by flow cytometry. Expression of BMP-4, -2 , twisted gastrulation and HPRT( house keeping gene) were measured via the quantitation of synthesized cDNA from RNA of stimulated B cells in the presence of sonic hedgehog proteins, using real-time PCR. Ct values from RT-PCR were used to draw a standard curve in order to measure the efficiency. after all, gene expressions were compared to HPRT expression by dividing the expression of each sample by the hprt values. graphs were plotted to analyse the difference between the expr3essions at 18 and 40 hrs.There was a decrease in TWG expression at 40 hrs and an increase of BMP-2 at 40 hrs which clearly supported the hypothesis. however BMP-4 expression significantly decreased at 40 hrs. The Idea is that Shh signalling increases BMP-2 expression at 40 hrs and has no effect on BMP-4. TWG expression was decreased at 40 hrs therefore it acted as an agonist of BMP-4 and antagonist of BMP-2.
Review and Research of Childhood Influences
Developing accounting information in conformity with generally accepted accounting principles is called financial accounting, because this information is designed to summarize the financial position and operating results of a business entity.
Differentiate between influences of heredity and environment on his/her psychological development. Ensure to specify which area of psychological development (moral, emotional, etc.).
Describe your experience with the Web-based EHR. What set of standards would apply to the Web-based EHR in acute or ambulatory care settings
The form of discrimination resulting from long-standign behaviors and practices accepted as the normal state of affaris or a reflection of reality is called?
Describe at least five components that should be incorporated in a formal disaster recovery plan in order that MailMed Inc. can become operational within 72 hours after a disaster affects its computer operations capability
Analyze whether or not the continuum of care services are adequate for the population with the chosen chronic illness/disability. Explain why or why not.
Outline the major arguments presented by the Metropolitan community Church challenging the traditional definition of marriage."
emembering that the acceleration due to gravity is -32 feet per second squared, how high is the stone 6 seconds later? At what time does the stone hit the ground?
Describe how the Sapir-Whorf hypothesis may affect communication across different cultures. Pay attention to the idea of symbols and symbolism between cultures.
A jet engine has ten components in series. The average reliability of each component is 0.998. What is the reliability of the engine?
What do Lindsey, Beauchamp, and Dick identify as a sufficient reason for a “scheme that allows physicians to provide legal assistance in hastening death” (383)?
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd