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Q. What differences in detail do you notice in freshly prepared versus permanently mounted tissue? How did the steps of preparations (with fresh microtome samples or staining and fixing of permanent mounts) affect the appearance of the samples? Tabulate some advantages and disadvantages of each. What component of root-tip cells is stained by Safranin in the permanent mount of a longitudinal section of a corn root tip? What component of root-tip cells does Fast Green stain? What could different stains such as these indicate with respect to cell division, differentiation, and function?
Glycogen is a polymer, so it has more energy than an equivalent number of glucose molecules. How does the cell find the energy necessary to make this storage product?
How many distinct molecular mechanisms might be responsible for converting a proto-oncogene into a potent oncogene?
How could you demonstrate experimentally that the selective antagonist binds the receptor specifically? Illustrate the experiment you would perform and also the controls you would contain.
Explain the roles of the CAP binding site and the operating site in relationship to the 3 proteins required for growth on lactose. Why aren't these proteins produced all the time? Explain the differences in function when glucose is absent and lact..
Explain the differences between eukaryotic and prokaroytic cells. Include in your discussion three phenotypic systems used to classify microbes.
What is the fate of pyruvate after it is formed by glycolysis? What is the overall reaction thermodynamics of glycolysis process?
you have purified DNA from you recently deceased goldfish. Which of the following restriction nucleases would you use if you wanted to end up with DNA fragments with an average size of 70 kilobase pairs (kb) after complete digestion of the DNA? Th..
If the light-dependent reactions in photosynthesis produce ATP and the mitochondria produce ATP during respiration, why do cells in the leaves of plants have both mitochondria and chloroplasts.
The foregut is the region that begins the digestive process by transforming large food chunks into a smaller chunky soup. The human stomach is a muscular sack of glands that secretes proteases and HCl.
Under which of the following conditions will the lac operon be expressed at the highest level?
How is the wavelength of light used to illuminate a specimen under a microscrope related to the power of resolution for a microscope?
Some people have extra copies of chromosome 21, 13, or 18. These extra chromosomes always result in traits not adaptable to current environment. Does this suggest that extra copies of chromosome 21, 13, or 18 are more toxic than extra copies of ot..
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