Method to use to check whether gene has been knocked out

Reference no: EM13526707

If you are asking which method to use to check whether the gene has been knocked out or not. This can be done by using gene expression. To know which method is used to generate a knockout mouse, it is RNAi also called RNA interference. I do understand this but i am asking Gene of unknown function in the mouse genome was investigated with reverse genetic approach Knock out mouse was created was missing particular gene When phenotype of investigated mouse was compared the only difference from the wild-type was mouse kicking other mouse And I know the protein encoded was named KST So my question is what are the some of aspects of the KST protien that could be investigated in order to better understanding the biology ?

Reference no: EM13526707

Questions Cloud

Dna-based test for the allele of a particular gene : A scientist discovers a DNA-based test for the allele of a particular gene. This and only this allele, if homozygous, produces an effect that results in death at or about the time of birth. Of the following, which is the best use of this discovery..

Phenotypic ratios would be expected : Radish flowers may be red, purple, or white. A cross between a red-flowered plant and a white-flowered plant yields all-purple offspring. The part of the radish we eat may be oval or long, with long being the dominant characteristic.

Gene of unknown function in the mouse genome : Gene of unknown function in the mouse genome was investigated with reverse genetic approach Knock out mouse was created was missing particular gene When phenotype of investigated mouse was compared the only difference from the wild-type was mouse kic..

Nucleotide polymorphisms used to determine genotype : How are single nucleotide polymorphisms used to determine genotype?

Method to use to check whether gene has been knocked out : If you are asking which method to use to check whether the gene has been knocked out or not. This can be done by using gene expression. To know which method is used to generate a knockout mouse, it is RNAi also called RNA interference.

Reaction mixture contains one microgram of enzyme : Find kcat for a reaction in which Vmax is 4 x 10^-4 mol•min-1 and the reaction mixture contains one microgram of enzyme (the molecular weight of the enzyme is 200,000 D).

Second-order reactions : Second-order reactions:

What are the subunits and functions of carbohydrates : What are the subunits and functions of carbohydrates? What are monosaccharides, disaccharides, and polysaccharides? What are the subunits and functions of lipids? Why is a fat a better storage compound than starch?

What are some possible genetic explanations : The gene (cv) is recessive and is known to be 13.7 Map units from the beginning of the chromosome. The recessive gene (f) is 51.7 map units from the beginning of the chromosome.

User Account

All Pages