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Question: Assume you are creating a PCR reaction mixture with a total volume of 50µL. The primer set concentration is 1µl primer/10µl reaction volume. The plasmid stock solution concentration = 0.01µg/µl, of which you need 100ng.How much of each PCR reaction component will you add, given the following?5x Mastermix __________Primer set __________DNA template __________H20 _____________
When chromatin from any eukaryote is digested with micrococcal nuclease and fractionated using electrophoresis, DNA fragments of approximately two hundred base pairs in length are observed.
Huntington disease is a rare dominant train auses neurodegeneration later in life. A man in his 30, who already has 3 children discoers his mother has huntingtons disease.
What percent of the population are homozygous dominant. What do you assume would be the effect on the switch of reversing orientation of one of the hix sites.
Compare the effects of this mutation with the effects of cholera toxin.why G-protein linked receptors activate G proteins.
These experiments demonstrated that DNA is the genetic material of phage and that protein does not transmit genetic information. Can this same experiment be carried out with 35S and 14C? Describe it.
LDL particles have a higher percent content of cholesterol and cholesterol esters than VLDL particles. Describe the catabolism of the VLDL particles that occurs in the circulation and how this accounts for the increased content of cholesterol in t..
Why benefits are strategically significant to employers and what is some main strategic consideration. What parts of the cell did molecules in this tube probably come from? Describe briefly.
Fossil with .9983 (Ur:Pb) in Africa
What is potentiation and how does it pose a threat to living organisms. what responsibility does carson believe scientists have to the public.
non covalent interactions which are most important. What is the driving force for proteins that are embedded in lipid membranes?
At what stage of the cell cycle are the chromosomes satisfactorily condensed that they FIRST become visible under the light microscope.
The sickle cell allele remains quite high populations of those with African Ancestry, and worldwide distribution of the sickle cell gene matches very closely to the worldwide distribution of Malaria.
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