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A student attempts to transform a population of E. coli with a pBluescript plasmid. How do you advise him to identify cells that have the plasmid? Answer Plate the cells on a medium containing X-gal. Cells with plasmids will turn blue. Plate the cells on a medium with ampicillin. Cells with plasmids will grow. Plate the cells on a medium with the restriction enzyme EcoRI. Cells with the plasmid will grow. Infect the cells with bacteriophage T3. Cells with the plasmid will transcribe the inserted gene and not be able to grow. Isolate individual colonies and prepare DNA from each. Size the DNA on an agarose gel to see if plasmids are present.
Suppose a fire hose ejects a stream of water at an angle of 38.8 ° above the horizontal. The water leaves the nozzle with a speed of 26.6 m/s.
In kumquat trees, plant height is determined by 3 genes as in our simplified model of polygenic inheritance. Each locus has a contributing allele and non-contributing allele.
The one hundred mile Leadville Marathon is being held on a very hot day and you are running the medical tent. A runner is brought in unconscious and with severe dehydration.
What are differences among eukaryotic cells . Why do you think she is having arrhythmias.
What is the function of science and technology in society. If you are in an airplane encountering turbulence and you start feeling sick to your stomach, would closing your eyes help and why.
Understand and discuss how the flow of nutrients differs from the dlow of energy in an ecosystem.
Josie has been taking steroids for a prolonged period of time as treatment for rheumatoid arthritis. She has gained weight, especially in her face, and notices that her skin is thin and bleeds easily.
An oligomeric protein has a molecular weight of 126000. A 820 mg sample was treated with an excess of 1-fluoro-2,4-dinitrobenzene until the reaction was finish.
What specific DNA sequence would you look for to determine if your mouse has the knockout gene, and what techniques would you use to detect this DNA sequence?
Researchers have adapted the principles of affinity chromatography to purify different types of protein, including lectin, recombinant protein and antibodies. Describe the ligand and matrix associated with isolating each of these three proteins.
Assume if the DNA in one of your skin cells were to change, which cells that are you would inherit that change?
What is the cellular/molecular mechanism to make yeast chemically competent? Compare and contrast the molecular mechanisms of yeast and bacterial transformations.
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