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Ion plays the role of hydrogen-ion donor

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Based on the equation below, which ion plays the role of hydrogen-ion donor (acid) and which ion plays the role of hydrogen-ion acceptor (base) in PBS?

 H2PO4-(aq) ?  H+(aq) + HPO42-

The composition of PBS is 0.137M NaCl, 0.012M Phosphate, 0.027M KCl, pH 7.4. Below is the protocol to make 1 liter of 10x concentrate PBS.

 Combine the following:

  • 80g NaCl
  • 2g KCl
  • 14.4g Na2HPO4(dibasic anhydrous)
  • 2.4g KH2PO4(monobasic anhydrous)
  • 800mL distilled H2O

1. Adjust pH to 7.4 with HCl

2. Add H2O to 1L

3. Autoclave for 20 minutes on liquid cycle. Store at room temperature.

Reference no: EM132186277

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