In recombination in bacteriophage genetics experiment

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In recombination in bacteriophage genetics experiment, instead of the mixed T4 rII phage strains being placed in a test tube containing KCN (potassium cyanide), the mixture is diluted 100 fold in 9.9mL of nutrient broth. What is the purpose of this dilution?

Note: I am referring to step 2 in the picture below, KCN was not added however and I am asking why did we dilute it 100 fold in nutrient broth anyway.

 

1982_Phage Recombination - p. 74.jpg

Reference no: EM131000278

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