Identify the protein that produced the pmf mass spectrum

Assignment Help Operation Management
Reference no: EM13286757

This assignment focuses on the methodology of peptide mass finger printing (PMF) and use of mass spectrum analysis software. The lecture notes provide a good foundation for this but you will have to carry out additional personal research to effectively answer the questions. The learning objective of the assignment is to give you an appreciation of the importance of bioinformatics and mass spectrometry in biomedical research technologies. Both mass spectrometry and bioinformatics are important skills in this area. 

Please complete the following tasks: 

The spectrum in the attached picture is a PMF profile obtained from a spot cut out of a 2 dimensional electrophoresis gel. 

1) Identify the protein that produced the attached PMF mass spectrum, you may use any online resource to do this but I suggest the MASCOT server is a good place to start. The profile was produced using trypsin and is derived from a human cell lysate. List the amino acid sequence of each fragment. 

2)  The MASCOT server lists a number of chemical modifications for peptides. Which of these are likely to have occurred due to the electrophoresis used to isolate the protein? What effect will these have on the protein’s mass? 

3) Using dot points, outline the processes used to produce the PMF profile starting from protein lysate.

Verified Expert

Reference no: EM13286757

What are optimal order quantity and the expected profit

The X Air Conditioning Company is considering purchase of a special shipment of portable air conditioners from Japan. Each unit will cost X $800 and be sold for $1250. X does

What is the approximate rate of return

X Company currently buys 6,500 units of a component part each year from a supplier for $7.70 each, but it is considering making the part instead. In order to make the part, X

Describe the sources of equity capital

Describe the sources of equity capital and the advantages/and disadvantages of each. Use one of each in a scenario. Describe the roles of private investors and venture capital

About environmental factors

Post about environmental factors which you think might call for more rigorous application of project management techniques, and factors you think might call for less rigorous

Identify the type of inventory system it uses

Use the library, corporate Web sites, and other online resources to locate a firm that incorporates the fixed order quantity system, fixed order period system, or a hybrid s

A company has recorded the last five days of daily

A company has recorded the last five days of daily demand on their only product. Those values are 120, 125, 124, 128, and 133. The time from when an order is placed to when

Sigma x chart control limits

Boxes of Honey Nut Oatmeal are produced to contain 16.0 ounces, with a standard deviation of 0.20 ounce. For a sample size of 49, the 3-sigma -x chart control limits are Upper

How construct a new factory would result

Not constructing a new factory would result in no additional revenue being generated because the current factories cannot produce these new products. Construct a decision tr

Reviews

Write a Review

 
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd