How would you elute the polypeptides

Reference no: EM13118732

Phenyl-Sepharose resin consists of a phenyl group attached to a matrix of agarose. Suppose you need to use this chromatographic method to separate a mixture of polypeptides consisting of polyalanine, polyserine, polytryptophan and polylysine. Would you apply these polypeptides in a buffer containing high ionic strength or low ionic strength? What buffer would you choose (choose from one of "Good's buffers" (find a list online)? How would you elute the polypeptides, and what would be the order of elution?

Reference no: EM13118732

Questions Cloud

Describes the structure of rna molecules in general : Which of the following statements best describes the structure of RNA molecules in general?

Explain what is the force felt by the electrons : Explain what is the force felt by the electrons and the nuclei in the rod when the external field described in the problem introduction is applied?

Find how many card holders should be sampled : It would like to be within plus or minus $55 of the true mean with a 95% confidence level. The standard deviation is thought to be $700. How many card holders should be sampled?

Show a constant cost monopoly firm maximizing : what would this price be in order to eliminate the deadweight loss. Show and explain. Why would the monopoly not charge this price? Show and explain.

How would you elute the polypeptides : What buffer would you choose (choose from one of "Good's buffers" (find a list online)? How would you elute the polypeptides, and what would be the order of elution?

What mass of nan3 must be used : Car air bags operate by the explosive reaction of NaN3: 2NaN ( ) 2Na( ) 3N ( ) 3 2 s s + g

Should people not get price-make it fair return if they win : Why do people buy lottery tickets for a grand prize of 20 million dollars when the probability of winning the grand prize is 1 in 175 million?

Find out the rotational inertia of this object : Find out the rotational inertia of this object (a) about an axis through the center of the triangle and perpendicular to its plane (b) and about an axis that passes through one vertex and the midpoint of the opposite side.

Why are many of the prolines hydroxylated : Why does the primary structure of collagen contain a glycine every third residue? Why are many of the prolines hydroxylated?

User Account

All Pages