Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
You are asked to run an SDS-PAGE analysis of samples from an affinity chromatography experiment
performed by your TA. A recombinant protein with a hexahistidine tag was captured in a nickel- agarose bead matrix and eluted off the column with 75mM imidazole. Your TA was hoping to make about 2 mg/mL of the protein. You are given the following samples to prepare and load into an SDS-PAGE gel :
· 10 uL 75 mM Imidazole
· 10 uL pure recombinant protein at 2 mg/mL
· 10 uL recombinant protein in 75 mM imidazole (elution fraction)
1. Describe how you would prepare the three samples to load into the gel.
2. Label the gel lanes with the appropriate contents. The recombinant protein should exhibit one band at 66,000 Daltons
3. Compare the elution lane to the positive control lane. How do you know the recombinant protein present in the elution sample? How pure is the elution sample compared to the control?
4. What assumption can you make about the amount of recombinant protein in the elution sample? What assay can you perform to find the accurate concentration of the elution sample?
A molecular level description means that you must explain the structure of water and how that structure leads to the observation made- in this case that water beads on waxes.
Noda and Numa in 1984 isolated the first voltage-gated sodium channel gene from the electric organ of the electric eel. Electric organs contain a high density of sodium channels. Tetrodotoxin (TTX) is a potent neurotoxin that blocks sodium channels. ..
Describe the process of muscle contraction and how a neuromuscular blocking agent, such as metubine, would interfere with muscle contraction.
Trace the pathway of blood through your body including the four chambers of the heart, four cardiac valves, aorta, vena cava, pulmonary arteries and veins, lugs, artery, arterioles, veins, venules, and capillaries.
G for complete oxidation of glucose is -686 Kcal/Mole. According the G for ATP and number of ATP's produced from the complete oxidation of glucose calculate the efficiency of biological oxidation of glucose by respiration.
The patient lives near the San Luis Rey River and has no history of foreign travel, blood transfusion, or intravenous drug use. What is the disease? How was it obtained.
A particular variety of corn has a gene for kernel color and a gene for height with the phenotypes CC,Cc: purple kernel.
In the F2 generation, how many genotypic classes are possible from a trihybrid cross of heterozygotes in Determine which the genes involved show complete dominance?
Who does not readily enter blood capillary of the villi but must enter the lymphatic capillary of villus instead, amino acid, gluclose, calcium and copper or cholesterol and triglyceride?
E valuate primary and secondary succession. Explain each type of succession thoroughly and give specific examples of everytype.
In an experimental population of Drosophila, a sample of males and virgin females includes 112 A1A1, 108 A1A2, and 30 A2A2 flies. Each genotype is represented equally in both sexes.
In lecture we discussed plants transitioning from water to land, thus pretend you were this plant clearly identify at least theree things you need to survive on land.
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd