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In a sample from a grasshopper population, you find four alleles at a particular locus. The frequencies of A1, A2, A3, and A4 are p1= 0.50, p2=0.30, p3=0.15, and p4=0.05. Assuming Hardy-Weinberg equilibrium, calculate the expected proportion of each of the ten possible genotypes (e.g., that of A2A3 should be 0.09). Show that heterozygotes, of all kinds, should constitute 63.9 percent of the population. In a sample of 100 specimens, how many would you expect to be heterozygous for allele A4? How many would you expect to be homozygous A4A4?
You genetically engineer nucleoplasmin so that a stretch of amino acids near its C-terminal end is removed. What happens to the resultant nucleoplasmin protein if this altered gene is transfected into an amphibian oocyte and transcribed into mRNA,
Which chemical modification do we associate with changing the activity of an enzyme in a transient/non-permanent manner?
Another dog, Billy, has three restriction sites for this enzyme in his genome. For this problem suppose there is only one chromosome in dogs.
calculate as many points on the curve as needed to discern its basic charcteristics.how to plot points on a graph
Difference between a hereditary germline cancer, such as those caused by BRCA1 mutations, and cancers that result from two somatic mutations in the same gene in the same cell.
Which minerals make up the majority of bone tissue, and what is their general structural purpose?
How do you know when you are looking at the epithelial vs. connective tissue? simple squamous vs. pseidostatified columnar? Contrast tissues of choice. Find similarities between tissues.
Which RNA polymerase is used in Lac operon expression? Which one is used in eukaryotic gene expression?
Assume you are creating a PCR reaction mixture with a total volume of 50µL. The primer set concentration is 1µl primer/10µl reaction volume.
Describe the mechanism of ELISA. Why is ELISA so sensitive? Why is it necessary to block unoccupied binding sites in the microtiter wells? Why is it important to have a positive control?
In the presence of high intracellular concentrations of tryptophan, only short transcripts of the trp operon are synthesized because of attenuation of transcription 5' to the structural genes.
The hormone insulin regulates glucose concentration in the blood by metabolic changes. Finish the flowchart below by moving the labels to the correct targets.
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