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Consider a culture into which two E. coli strains are inoculated. Strain A is normal with g=21 min at 37 degrees C, and 42 mins at 27 degrees C. Strain B has a temperature-sensitive mutation, such that g=55 mins at 37 degrees C, and 39 mins at 27 degrees C. We inoculate equal amounts of cells (1 ml of each starter culture whose population is 1 x 10^6 cfu/ml) into each of two separate 1 liter volumes of LB broth at time=0. One of the flasks is incubated at 37 degrees C, one at 27 degrees C.
Assuming constant exponential growth (i.e. no lag phase, and no exhaustion of nutrients), answer each of these questions:
1) After 6 hours of growth, what is the final concentration of each strain in each flask?
2) What fraction of the total population is Strain A and Strain B in each flask?
3) How long would each flask need to go for one strain to become 90% of the population?
Describe what you observed in your plaque smear wet mount, direct stained slide and indirectly stained slide. What were the similarities? Describe the three main steps to clone a gene into an organism.
In D. Melanogaster cherub wings(ch), black body (b) and cinnabar eyes (cn) result from recessive alleles tht are all located on chromosome 2. A homozygous wild-type fly was mated with a cherub, black, and cinnabar fly.
A continuously growing population has a population size of 1,000 and an intrinsic rate of increase of r = 0.05 per year. Assuming that this rate of increase remains the same, about how long should it take for the population to reach 4,000?
What are the equilibrium concentrations of fructose 1,6-biphosphate, dihydroxyacetone phosphate and glyceraldehyde 3-phosphate when 1mM fructose 1,6-biphosphate is incubated with aldolase under standard conditions?
If the chromosomes are 47 and chromosomal disorder is super female what could be the analysis for this kind of karotype including the symptoms of the condition.
A profressor in a second-term calculus course wants to determine whether the year in college has any effect on the performance of his students on their final exam.
Select a specific gene and a specific alteration. Explain the effects of the modifications and why it might contribute to the cancer.
As you know Griffiths, and Avery, MacLeod and McCarty 1st experimented with the concepts of lateral gene transfer in bacteria as it relates to transformation.
Determine which of the following molecules or substances contain, or are derived from, fatty acids?
Which type(s) of progeny will be produced when ovules from a green four o'clock plant are fertilized with pollen from a variegated plant.
Which of the follow statements regarding cancer risk factors is false. Mutagens are generally not carcinogens.
What progeny would result from crossing a pure-bred rose combed hen with dihybrid walnut rooster? Give the genotypes of the parents and the genotpye and phenotypes of the F1.
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