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Consider a culture into which two E. coli strains are inoculated. Strain A is normal with g=21 min at 37 degrees C, and 42 mins at 27 degrees C. Strain B has a temperature-sensitive mutation, such that g=55 mins at 37 degrees C, and 39 mins at 27 degrees C.
We inoculate equal amounts of cells (1 ml of each starter culture whose population is 1 x 10^6 cfu/ml) into each of two separate 1 liter volumes of LB broth at time=0. One of the flasks is incubated at 37 degrees C, one at 27 degrees C.
Assuming constant exponential growth (i.e. no lag phase, and no exhaustion of nutrients), answer each of these questions:
1) After 6 hours of growth, what is the final concentraion of each strain in each flask?
2) What fraction of the total population is Strain A and Strain B in each flask?
3) How long would each flask need to go for one strain to become 90% of the population?
Give an example of an area of research and science which requires a code of ethics when suggesting your modifications to the scientific method.
A nine year-old boy decided to find out how long he could breathe into and out of a paper bag. After ~2 minutes his friends noticed that he was breathing very rapidly.
What happens to the post-synaptic cells' membrane potential at an inhibitory synapse?define why the coupling of the import of amino acids.
Determine what is known mechanistically about how dosage compensation occurs in mammals/humans? what gene is involved with the process?
What does it mean when someone talking about poultry genetics and the say parent stock?
Consider on all the things you learned about sex & sexuality while increasing from sources such as family, school, friends, TV, movies, music, magazines, Internet, and boy/girlfriends.
Based on the dimensions of an alpha-helix, compute the range in width of the membrane? Discuss how many amino acids would be needed in an antiparallel beta strand to span the membrane?
Determine which of the following are features of allosteric inhibition? During the infection, there are many different environments that P. multocida will encounter.
A dog homozygous for both dominant traits is mated to a droopy-eared silent follower. The expected phenotypic ratio and genotypic ratio in the F-1 generation.
Robert, a smoker, is 52 years old. He has elevated blood pressure, in part, due to an elevated erythrocyte count. Knowing that smokers often have reduced plasma O2 levels, explain in detail (in a paragraph) why his erythrocyte count is elevated.
In Drosophila melanogaster you are studying three phenotypes that you suspect are linked on the same chromosome. THe homozygous recessive traits for each locus are.
What aspects of the native organ function are replicated, and which are not? (this should include a quantitative analysis of function, for example filtration rates in artificial kidney compared to natural kidney)
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