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1. How are cloning vectors then put into bacteria cells?
2. How do we know if the bacteria cell has the vector? How do we know if the vector contains the insert?3. How is a cloning vector different from an expression vector?4. How does a Eukaryotic gene have to be modified before being expressed by a bacteria cell?
DNA footprinting allows isolation of DNA sequences that bind specific transcription factors. Describe an experimental protocol to identify transcription factors that bind to an isolated DNA sequence.
The "Primordial soup" includes the mixture of elements and gases which are thought to have started life on the Earth. Specify where did the gases and elements come from?
A cell whose cytoplasm has a concentration of 0.02 molar glucose is placed in a test tube of water containing 0.02 molar glucose. Assume that glucose is not actively transported into the cell, which terms describes the tonicity of an external solutio..
What information would you get from a western blot that you would not get from a northern blot when used to analyze the expression of the same gene?
q1. a woman without hypophosphatemia and a man with hypophosphatemia have a daughter. the daughter mates with a male
Consider the three major classes of biologically important molecules: proteins, lipids, and nucleic acids. Which steps of the miniprep procedure act on proteins?
A mutant prostist is found in which some mitochondria lack an inner mitochondrial membrane. Which pathways would be completely disrupted in these mitochondria.
the acquired antibiotic resistance genes encode what types of proteins that can inhibit the function of antibiotic resistance?
Describe how a linear sequence of amino acids gives rise to the three-dimensional structure of proteins. Detail the significance of this structure, how it may be modified, and the results of such modification.
cells can be categorized as either prokaryotic or eukaryotic. only bacterial cells are prokaryotic. for question two
what are the chemical journey's of a carbon atom from carbon dioxide in the air and a hydrogen atom from a water molecule that have entered a plant cell and what are their chemical journeys as they are involved in in the process of photosynthesis ..
Describe how bone is maintained and modeled by hormonestimulation. Include osteoclasts, osteoblasts, the hormones involvedand their effects
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