Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
2. You isolate a Gram positive alkalinophile that grows at a pH = 10. At this external pH, the intracellular pH is maintained at 7.3. Using the equation proton motive force (pmf), determine the contributions of the electrical and chemical energy components to the pmf in this bacterium. 3. You are studying the type VI secretion system in Pseudomonas aeruginosa. You analyze the genome and find an operon comprised of 5 genes that you believe is involved building the secretion apparatus. You name the genes tssA, tssB, tssC, tssD, and tssE based simply on their order. You believe that tssA(the first gene in the operon) encodes the crucial component of the secretion apparatus. Design an experiment to make a knockout of tssA. How will you determine if the mutation in tssA is a polar mutation? 4. You obtain a clinical isolate of the Gram positive pathogen Clostridium difficile that is highly resistant to vancomycin. This is a significant health concern as vancomycin is a 'last line of defense' for treatment of C. difficile infections. You notice that all of the clinical isolates were taken from patients suffering from a multispecies infection, including a vancomycin resistant strain of E. faecalis. What couldexplain this? State your hypothesis and develop experiments to test it. 5. You treat 107Pseudomonas aeruginosa growing planktonically and as a biofilm with high levels of the antibiotic tobramycin (10 micrograms/ml) for 2 hours. No surviving planktonic bacteria are recovered, although 104 biofilm bacteria survive this treatment. Design an experiment to identify genes important for biofilm resistance in P. aeruginosa.
Iodoacetate reacts with cysteine side chains in proteins to form S-carboxymethyl derivatives. Why is it a good idea to treat a protein first with beta-mercaptoethanol before carrying out carboxymethylations of cysteine residues? Why might treatmen..
Discuss the principle of segregation? Explain why is it important? Determine the concept of dominance? How does dominance differ from incomplete dominance.
What is the pattern of radioactivity in the sister chromatids after the first and second round of DNA replication ?
A 53 firefighter suffers third degree burns over her hands. Which of the following cell types is primarly responsible for contractues that may develop in this patinet?
Complete the table by writing down the genotype of the parents or parents involved in each cross. Utilize the letter P for purple and p for white. What are the genotypes of the parent plants.
In many countries, plants face flooding stress especially around river basins, and due to flash floods, typhoons. Few days of complete submergence will negatively impact the growth and development of plants.
Coat color in mice is determined by two independently assorting but interacting genes. The dominant allele at the Black locus (B) results in a black pigment being deposited in the fur; the recessive allele (b) leads to no pigment being made and an..
Two populations of elk have exactly the same total population sizes and identical age-specific schedules of birth and death rates, and yet these two populations are currently growing at different rates. How can this be?
Which of the three predator functional responses would you expect to be most likely to give stable populations, and why?
Use chi-square test, test whether these two loci show independent assortment? How would you explain the frequency of the progeny genotypes?
explain the feedback mechanism that maintain homeostasis and give two specific examples of what happen when homeostatic control systems do not function properly.
How is it that different cells can respond in different ways to exactly the same signaling molecule even when they have identical receptors?
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd